Table 1.

Sample of de novo synthesized proteins isolated from stably transfected HEK293 cells expressing hSloα after 6 h ethanol incubation^{^a}

Protein	Gene _human	Accession number	Biological function	EtOH	Naive	Null	EtOH/naive
Ca²⁺activated K channel subunit α-1	KCNMA1	Q28204	Potassium channel activated by both membrane depolarization or increase in cytosolic Ca²⁺ that mediates export of K⁺	13	15	3	0.9
Catenin β-1	CTNB1	P35222	Key downstream component of the canonical Wnt signaling pathway	14	7	0.5	2.0
Spectrin β chain	SPTB2	Q01082	Involved in secretion, interacts with calmodulin in a calcium-dependent manner, and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane	22	9	7	2.4
Amyloid-like protein 2	APLP2	Q06481	May play a role in the regulation of hemostasis; the soluble form may have inhibitory properties toward coagulation factors	10	6	2	1.7
Clathrin interactor 1	EPN4	Q14677	Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2); may have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes; stimulates clathrin assembly	9	3	1	3.0
Junction plakoglobin	PLAK	P14923	Common junctional plaque protein; can replace β-catenin in E-cadherin/catenin adhesion complexes, which are proposed to couple cadherins to the actin cytoskeleton	32	21	14	1.5
mRNA export factor	RAE1L	P78406	Binds mRNA; may function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton	6	4	0	1.5
Coatomer subunit β	COPB	P53618	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non–clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans-Golgi network	12	5	1	2.4
Polyadenylate-binding protein 1	PABP1	P11940	Binds the poly(A) tail of mRNA	35	28	9	1.3
Clusterin	CLUS	P10909	Extracellular chaperone that prevents aggregation of non-native proteins	6	4	0	1.5
Probable ATP-dependent RNA helicase DDX5	DDX5	P17844	Involved in the alternative regulation of pre-mRNA splicing	34	27	7	1.3
BCL-6 corepressor	BCOR	Q6W2J9	Transcriptional corepressor	6	2	0	3.0

↵^aSummed unique peptide count control (naive), 25 mM ethanol and null (no AHA control) all after 6 h incubation. Ratio of ethanol to naive highlights fold differences in peptide detection for each protein.