Corelease of glutamate and GABA from the mossy fibers restrains the number of action potentials and instantaneous fire frequency of regular spiking, stratum lucidum interneurons in a frequency-dependent manner (red traces in Fig. 6)
| T1 | T2 | T3 | |
|---|---|---|---|
| No stimulation | MF stimulation | No stimulation | |
| MF stim (Glu/GABA correlease) | |||
| AP number | 45 ± 5 | 30 ± 3* | 22 ± 3* |
| IF Freq (Hz) | 14.2 ± 2 | 10.3 ± 1 | 8.4 ± 1 |
| 20 Hz stimulation | |||
| AP number | 36 ± 10 | 24 ± 12* | 48 ± 12* |
| IF Freq (Hz) | 12.6 ± 3 | 1.1 ± 1.5* | 11.8 ± 1.4* |
| 40 Hz stimulation | |||
| AP number | 33 ± 7 | 2 ± 1** | 55 ± 7** |
| IF Freq (Hz) | 20.5 ± 3.2 | 0.94 ± 0.6** | 14.7 ± 1.8* |
| MF Stim (Glu only) | |||
| AP number | 51 ± 4 | 26 ± 3* | 23 ± 3* |
| IF Freq (Hz) | 20.9 ± 7.1 | 12.43 ± 2.4 | 11.7 ± 1.2 |
| 20 Hz stimulation | |||
| AP number | 38 ± 5 | 45 ± 4* | 33 ± 4 |
| IF Freq (Hz) | 18.5 ± 4 | 24.5 ± 4* | 15 ± 2.4 |
| 40 Hz stimulation | |||
| AP number | 30 ± 4 | 50 ± 1* | 21 ± 4 |
| IF Freq (Hz) | 24.13 ± 4 | 40.5 ± 2.2** | 19 ± 3.3 |
By contrast, interneurons receiving an exclusively glutamatergic input (blue traces in Fig. 6) fire AP in a frequency-dependent manner. AP, Action potential; IF Freq, instantaneous fire frequency; MF stim, MF stimulation; T, time window. The values were obtained from the traces depicted in Fig. 6.
↵*p < 0.05.
↵**p < 0.01.