Table 1.

Summary of unbiased disector counts of synapses made by VGluT2+ boutons in M1 and S1 L4 for 3 mice

MouseM1S1
No. of synapsesSynapses density per μm3 ± SEM (NVVGluT2 and NVasym)Percentage of VGluT2 synapsesNo. of synapsesSynapse density per μm3 ± SEM (NVVGluT2 and NVasym)Percentage of VGluT2 synapses
1VGluT2: 85 (218)VGluT2: 0.156 ± 0.01810.1%VGluT2: 162 (316)VGluT2: 0.205 ± 0.01716.1%
all asym: 594 (154)all asym: 1.543 ± 0.059all asym: 724 (228)all asym: 1.270 ± 0.046
2VGluT2: 45 (82)VGluT2: 0.220 ± 0.03314.2%VGluT2: 46 (80)VGluT2: 0.230 ± 0.03819.9%
all asym: 278 (72)all asym: 1.544 ± 0.080all asym: 208 (72)all asym: 1.156 ± 0.083
3VGluT2: 44 (88)VGluT2: 0.200 ± 0.03011.9%VGluT2: 48 (80)VGluT2: 0.240 ± 0.03615.6%
all asym: 310 (74)all asym: 1.676 ± 0.080all asym: 261 (68)all asym: 1.535 ± 0.084
12.1%17.2%
  • We counted VGluT2+ and unlabeled asymmetric synapses (“all asym” is the sum of labeled and unlabeled synapses) in a large number of disectors (indicated in parentheses). Then, we derived the density of VGluT2+ synapses (NVVGluT2) and all asymmetric synapses (VGluT2+ plus unlabeled, NVasym) by dividing the synapse count by the respective number of disectors analyzed, and by the disector volume (2.5 μm3). Synapse densities are given with SEM. Finally, we calculated the percentage of VGluT2+ synapses out of all asymmetric synapses by dividing NVVGluT2 by NVasym.