Table 1.

List of antibodies used in this study

Primary antibodiesCatalog numberIncubationSecondary antibodies
eIF4EPhospho-9741Sp, 1:500; p(IHC), 1:1000; t, 1:1000; overnight at 4°CGoat anti-rabbit (Western blot, 1:10,000; IHC, 1:1000); 1 h at RT
Phospho (IHC)-ab76256
Total-9742S
4E-BP1Phospho-9459Sp, 1:500; t, 1:1000; overnight at 4°CGoat anti-rabbit (1:10,000); 1 h at RT
Total-9452S
ERKPhospho-9101Sp, 1:3000; t, 1:3000; overnight at 4°CGoat anti-rabbit (1:10,000); 1 h at RT
Total-9102S
GAPDH21181:10,000; overnight at 4°CGoat anti-rabbit (1:10,000); 1 h at RT
PeripherinSAB 45024191:500; overnight at 4°CAlexa Fluor goat anti-rabbit 488 (1:1000); 1 h at RT
Neurofilament 200N01421:400; overnight at 4°CAlexa Fluor goat anti-mouse 568 (1:1000); 1 h at RT
IB4−568I21412Spinal cord, 1:1000; DRG, 1:400; overnight at 4°CNone
CGRPT-4032Spinal cord and DRG, 1:1000; overnight at 4°CAlexa Fluor goat anti-rabbit 647 (1:2000); 1 h at RT
TRPV1GP14100Spinal cord and DRG, 1:1000; skin, 1:3000; overnight at 4°CAlexa Fluor goat anti-guinea pig 488 (1:2000); 1 h at RT
  • RT, Room temperature; t, total.