Primary antibodies | Catalog number | Incubation | Secondary antibodies |
---|---|---|---|
eIF4E | Phospho-9741S | p, 1:500; p(IHC), 1:1000; t, 1:1000; overnight at 4°C | Goat anti-rabbit (Western blot, 1:10,000; IHC, 1:1000); 1 h at RT |
Phospho (IHC)-ab76256 | |||
Total-9742S | |||
4E-BP1 | Phospho-9459S | p, 1:500; t, 1:1000; overnight at 4°C | Goat anti-rabbit (1:10,000); 1 h at RT |
Total-9452S | |||
ERK | Phospho-9101S | p, 1:3000; t, 1:3000; overnight at 4°C | Goat anti-rabbit (1:10,000); 1 h at RT |
Total-9102S | |||
GAPDH | 2118 | 1:10,000; overnight at 4°C | Goat anti-rabbit (1:10,000); 1 h at RT |
Peripherin | SAB 4502419 | 1:500; overnight at 4°C | Alexa Fluor goat anti-rabbit 488 (1:1000); 1 h at RT |
Neurofilament 200 | N0142 | 1:400; overnight at 4°C | Alexa Fluor goat anti-mouse 568 (1:1000); 1 h at RT |
IB4−568 | I21412 | Spinal cord, 1:1000; DRG, 1:400; overnight at 4°C | None |
CGRP | T-4032 | Spinal cord and DRG, 1:1000; overnight at 4°C | Alexa Fluor goat anti-rabbit 647 (1:2000); 1 h at RT |
TRPV1 | GP14100 | Spinal cord and DRG, 1:1000; skin, 1:3000; overnight at 4°C | Alexa Fluor goat anti-guinea pig 488 (1:2000); 1 h at RT |
RT, Room temperature; t, total.