Regular Article
A Novel ES Cell Line, TT2, with High Germline-Differentiating Potency

https://doi.org/10.1006/abio.1993.1458Get rights and content

Abstract

In producing mutant mice by gene-targeting and gene-trapping in embryonic stem (ES) cells, the efficient colonization of the mutant ES cells into germline is still a critical matter. We have established a new line of ES cells, TT2, from an Fl embryo between a C57BL/6 female and a CBA male. When the TT2 cells were injected into blastocysts, the colonization into each tissue was very low. However, when injected into eight-cell embryos, the cells segregated inside the blastomeres, localized in an inner cell mass of blastocysts developed 1 day later, and colonized efficiently in each tissue of the pups. The pups were disproportionately male, about half of which were composed of TT2-derived cells primarily; in more than 70% of the males, TT2-derived cells were dominant, accounting for over half of the total cells. When these males were mated, they exclusively yielded TT2-derived offspring. The germline-differentiating potency was stable during 3 weeks of culture. Twenty-one of 24 mutant clones independently isolated yielded germline chimeras, and 19 clones yielded them in a rate comparable to that of the parent cells. Thus, TT2 cells can serve as a valuable vehicle for the production of mutant mice.

References (0)

Cited by (441)

  • GAREM1 is involved in controlling body mass in mice and humans

    2022, Biochemical and Biophysical Research Communications
    Citation Excerpt :

    The processed samples were analyzed as described previously [1,2]. The GAREM1 conditional KO mice (Accession No. CDB1257K; https://large.riken.jp/distribution/mutant-list.html) were generated using TT2 ES cells [14] as described (https://large.riken.jp/protocol/others.html). The mouse GAREM1 gene comprises six exons located in chromosome 18.

  • USP40 deubiquitinates HINT1 and stabilizes p53 in podocyte damage

    2022, Biochemical and Biophysical Research Communications
View all citing articles on Scopus
View full text