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TRAIL Causes Cleavage of Bid by Caspase-8 and Loss of Mitochondrial Membrane Potential Resulting in Apoptosis in BJAB Cells

https://doi.org/10.1006/bbrc.1999.1641Get rights and content

Abstract

A new member of the TNF family, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), has been shown to induce apoptosis. However, the mechanism for TRAIL-induced apoptosis remains to be clarified. SDS-PAGE and Western blot analysis showed that cleavage of Bid was induced by a 1-h incubation of BJAB cells with TRAIL and was blocked by a caspase-8 inhibitor. Flow cytometry demonstrated that loss of mitochondrial membrane potential in BJAB cells began about 1.5 h after the treatment with TRAIL and was apparent at 2 h in comparison with the control. DNA ladder formation, which is characteristic for apoptosis, in the cells treated with TRAIL was detected at 2 h and observed most effectively at 3 h. The time course study suggests that TRAIL causes cleavage of Bid via activation of caspase-8, subsequently the loss of mitochondrial membrane potential, resulting in apoptosis in BJAB cells.

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    Abbreviations used: Ac-IETD-CHO, acetyl-lisoleucyl-l-glutamyl-l-threonyl-l-aspart-1-aldehyde; Ac-DMQD-CHO, acetyl-l-aspartyl-l-methionyl-l-glutaminyl-l-aspart-1-aldehyde; DiOC6(3), 3,3′-dihexyloxacarbocyanine iodide; CHX, cycloheximide; FCS, fetal calf serum; FACS, fluorescence-activated cell sorting.

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