Journal of Molecular Biology
Kinetics of Expression of Inducible β-Galactosidase in Murine Fibroblasts: High Initial Rate Compared to Steady-state Expression
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Mechanical regulation of PTHrP expression in entheses
2007, BoneCitation Excerpt :We approached unloading of selected entheses using tail suspension or surgical transection of the tendon or ligament in question. The t½ of β-galactosidase (β-gal) has been reported in the literature as 8, 13 and 43 h, for a mean of about 24 h [14–16]. Using this figure, one would achieve 1/8 of an initial signal at 72 h (assuming complete shut down), so that this was chosen as our initial minimum period of post-manipulation observation with each technique.
Spatiotemporal expression and localization of matrix metalloproteinas-9 in a murine model of thoracic aortic aneurysm
2006, Journal of Vascular SurgeryCitation Excerpt :To determine the localization of active MMP-9 transcription, β-gal activity in explanted aortas was identified in situ by reaction with the colorimetric substrate X-gal. Because the β-gal enzyme has a very short half-life upon translation, estimated at 13 hours in fibroblasts,23 X-gal staining reveals recent transcriptional activity within the tissue samples, thus allowing quantitation and localization simultaneously. The results demonstrated that β-gal was clearly elevated within the aortic adventitia at 2 weeks post-TAA induction.
Gene delivery using temperature-responsive polymeric carriers
2002, Drug Discovery TodayCitation Excerpt :First, specific promoters [2] can start the selective transcription of the encoded gene in specific environments. Physical signals (γ-ray [3,4] and temperature [5]) and chemical signals, such as lactose [6] and tetracycline [7,8], induce transcription of the genes placed downstream from the signal-inducible promoters. Alternatively, tissue- or organ-specific promoters have been used to obtain the corresponding gene expression even if signal species are not identified in some cases.
Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling
2020, Frontiers in Cell and Developmental BiologyEpibranchial placode-derived neurons produce BDNF required for early sensory neuron development
2011, Developmental Dynamics
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