Elsevier

Methods

Volume 10, Issue 3, December 1996, Pages 289-291
Methods

Regular Article
Gene Delivery into Neuronal Cells by Calcium Phosphate-Mediated Transfection

https://doi.org/10.1006/meth.1996.0105Get rights and content

Abstract

This paper describes a method for introducing DNA constructs into primary adult dorsal root ganglion (DRG) neuron cultures. The method uses a modified calcium phosphate technique and enables relatively small numbers of cells to be used. We have used this method to study the promoter sequences responsible for mediating gene activity and nerve growth factor responsiveness in DRG neurons. It can also be used, however, for other purposes such as testing the effect on neuronal function of overexpressing a specific gene product.

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1

Present address: Eisai London Research Laboratories, Bernard Katz Building, University College London, Gower Street, London WC1E 6BT.

2

To whom correspondence should be addressed. Fax: 44-171-387-3310.

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