Regular ArticleCollagen Gel Coculture of Neural Tissue
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GAP-43 dependency defines distinct effects of netrin-1 on cortical and spinal neurite outgrowth and directional guidance
2013, International Journal of Developmental NeuroscienceCitation Excerpt :Its function in chemotropic guidance has been quantified in co-culture experiments by determining the sidedness of neurite outgrowth toward a cellular source of netrin-1 (Guthrie and Lumsden, 1994; Kennedy et al., 1994). Fig. 1e–h demonstrates that netrin-1-mediates chemotropic guidance in these co-cultures using the criteria established by Guthrie and Lumsden (1994). Section 2 provides details of how this chemotropic guidance was quantitated.
Localized gene expression of axon guidance molecules in neuronal co-cultures
2007, Journal of Neuroscience MethodsIn Vitro and in Vivo Characterization of a Novel Semaphorin 3A Inhibitor, SM-216289 or Xanthofulvin
2003, Journal of Biological ChemistryCitation Excerpt :To assess if SM-216289 has the potential to inhibit the redirection of growing axons by Sema3A, we employed a collagen gel co-culture system. When neurons are co-cultured in a collagen gel matrix very close to cells producing repulsive or attractive guidance molecules, such as a semaphorin or netrin, growing neurites sense the concentration gradient and change their direction of growth accordingly (34). As shown in Fig. 2, an aggregate of Sema3A-expressing COS7 cells (Sema3A-COS) and E8 chick DRG explant were co-cultured in a collagen gel for 2 days with or without SM-216289 in the growth medium.
Signals from the cerebellum guide the pathfinding of inferior olivary axons
2003, Developmental BiologyPatterning of motor neurons by retinoic acid in the chick embryo hindbrain in vitro
2003, Molecular and Cellular Neuroscience