Summary
A quantitative description of motor seizures induced by pentylenetetrazol (PTZ, metrazol) was performed. Seizures were induced by PTZ in doses from 40 to 120 mg/kg s.c. in 477 male albino rats of the Wistar strain 7 to 90 days old. Two patterns of seizures were elicited: minimal, i.e. predominantly clonic seizures of facial and forelimb muscles with preserved righting ability, and major, i.e. generalized tonic-clonic seizures with a loss of righting reflex. Minimal seizures could be reliably elicited since the age of 18 days; the CD50 for these seizures did not significantly differ with age. Major seizures were elicited regularly at all developmental stages studied. Their CD50 did not significantly differ among 7-, 12- and 25-day-old rat pups but the value for 18-day-old rats was smaller and for adult animals larger than for these three age groups.
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References
Albala BJ, Moshe SL, Okada R (1984) Kainic acid-induced seizures: A developmental study. Dev Brain Res 13:139–148
Brink JJ, Cariglia N, Stein D, Galipeau LA (1970) Uptake of [14C] pentylenetetrazol by developing rat brain. Brain Res 19:445–450
Browning RA (1985) Role of the brain stem reticular formation in tonic-clonic seizures: lesion and pharmacological studies. Fed Proc 44:2425–2431
Browning RA, Nelson DK (1986) Modification of electroshock and pentylenetetrazol seizure pattern in rats after precollicular transections. Exp Neurol 93:546–556
de Casrilevitz M, Engelhardt E, Esberard CA (1971) Maturation of convulsogenic activity induced by leptazol in the albino rat. Br J Pharmacol 42:31–42
de Feo MR, Mecarelli O, Ricci GF (1985) Bicuculline- and allylglycine-induced epilepsy in developing rats. Exp Neurol 90:411–421
Gale K (1988) Progression and generalization of seizure discharge: Anatomical and neurochemical substrates. Epilepsia 29 [Suppl 2]:S15-S34
Haberer LJ, Pollack GM (1991) Central nervous system uptake kinetics of pentylenetetrazol in the developing rat. Biopharm Drug Disp 12:59–71
Levy RH, Dreifuss FE, Mattson RH, Meldrum BS, Penry JK (1989) Antiepileptic drugs, 3rd edn. Raven Press, New York
Löscher W, Schmidt D (1988) Which animal models should be used in the search for new antiepileptic drugs? A proposal based on experimental and clinical considerations. Epilepsy Res 2:145–181
Mareš P, Schickerova R (1980) Seizures elicited by subcutaneouos injection of metrazol during ontogenesis in rats. Activ Nerv Super 22:264–268
Mareš P, Trojan S (1991) Ontogenetic development of isonicotinehydrazide-induced seizures in rats. Brain Dev 13:121–125
Mareš P, Velisek L (1983) Influence of ethosuximide on metrazol-induced seizures during ontogenesis in rats. Activ Nerv Super 25:295–298
Miller RG (1981) Stimultaneous statistical inference. Springer, New York Berlin Heidelberg
Pohl M, Mareš P (1987) Flunarizine influences metrazol-induced seizures in developing rats. Epilepsy Res 1: 302–305
Reinhard JF, Reinhard JF Jr (1977) Experimental evaluation of anticonvulsants. In: Vida JA (ed) Anticonvulsants. Academic Press, New York, pp 57–111
Swinyard EA, Woodhead JH, White HS, Franklin MR (1989) Experimental selection, quantification, and evaluation of anticonvulsants. In: Levy RH, Dreifuss FE, Mattson RH, Meldrum BS, Penry JK (eds) Antiepileptic drugs, 3rd edn. Raven Press, New York, pp 85–102
Veliskova J, Velisek L, Mareš P (1988) Epileptic phenomena produced by kainic acid in laboratory rats during ontogenesis. Physiol Bohemoslov 37:395–405
Veliskova J, Velisek L, Mareš P, Rokyta R (1990) Ketamine suppresses both bicuculline- and picrotoxin-induced generalized tonic-clonic seizures during ontogenesis. Pharmacol Biochem Behav 37:667–674
Vernadakis A, Woodbury DM (1969) The developing animal as a model. Epilepsia 10:163–178
Yonekawa WD, Kupferberg HJ, Woodbury DM (1980) Relation between pentylenetetrazol-induced seizures and brain pentylenetetrazol levels in mice. J Pharmacol Exp Ther 214:589–593
Zouhar A, Mareš P, Liskova-Bernaskova K, Mudrochova M (1989) Motor and electrocorticographic epileptic activity induced by bicuculline in developing rats. Epilepsia 30:501–510
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Velisek, L., Kubova, H., Pohl, M. et al. Pentylenetetrazol-induced seizures in rats: an ontogenetic study. Naunyn-Schmiedeberg's Arch Pharmacol 346, 588–591 (1992). https://doi.org/10.1007/BF00169017
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DOI: https://doi.org/10.1007/BF00169017