Summary
Avian pancreatic polypeptide (APP)-like, molluscan cardioexcitatory peptide (FMRF)-like and neuropeptide Y (NPY)-like immunoreactivities were studied in a secondary visual pathway in rat brain. The cell bodies of this pathway are located in the lateral geniculate nucleus and its terminal plexus is found in the suprachiasmatic hypothalamic nucleus (SCN). The neurons and terminal plexus demonstrated by antiserum to each peptide are identical, and immunoreactivity is blocked by preabsorption of each antiserum with a low concentration of the antigen against which it was raised. Immunoreactivity is also blocked by preabsorption of each antiserum with either NPY or APP. In contrast, APP- and NPY-like immunoreactivities are blocked only partially when these antisera are preabsorbed with concentrations of FMRF as high as 100 μM. Since NPY is the only one of these peptides that has been isolated from mammalian brain, we conclude that NPY is the endogenous CNS peptide produced by neurons of the lateral geniculate-SCN projection.
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References
Adams JC (1977) Technical consideration on the use of horseradish peroxidase as a neuronal marker. Neuroscience 2:141–145
Allen YS, Adrian TE, Allen JM, Tatemoto K, Crow TJ, Bloom SR, Polak JM (1983) Neuropeptide Y distribution in the rat brain. Science 221:877–879
Boer HH, Schot LPC, Veenstra JA, Reichelt D (1980) Immunocyto-chemical identification of neural elements in the central nervous system of a snail, some fish, and a mammal with antiserum to the molluscan cardioexcitatory tetrapeptide FMRF-amide. Cell Tissue Res 213:21–27
Card JP, Moore RY (1982) Ventral lateral geniculate nucleus efferents to the rat suprachiasmatic nucleus exhibit avian pancreatic polypeptide-like immunoreactivity. J Comp Neurol 206:390–396
Card JP, Brecha N, Karten HJ, Moore RY (1981) Immunocyto-chemical localization of vasoactive intestinal polypeptide containing cells and processes in the suprachiasmatic nucleus of the rat: Light and electron microscopic analysis. J. Neurosci 1:1289–1303
Card JP, Brecha N, Moore RY (1983) Immunohistochemical localization of avian pancreatic polypeptide-like immunoreactivity in the rat hypothalamus. J Comp Neurol 217:123–136
Dockray GJ, Williams RG (1983) FMRF-amide-like immunoreactivity in rat brain: Development of a radioimmunoassay and its application in studies of distribution and chromatographic properties. Brain Res 266:295–303
Dockray GJ, Vailant C, Williams RG, Gayton RJ, Osborne NM (1981) Vertebrate brain-gut peptides related to FMRFamide and Met-enkephalin Arg6 Phe7. Peptides 2: Suppl 2:25–30
Gayton RJ (1982) Mammalian neuronal actions of FMRFamide and the structurally related opioid Met-enkephalin-Arg6-Phe7. Nature 298:275–276
Hickey TL, Spear PD (1976) Retinogeniculate projections in hooded and albino rats: An autoradiographic study. Exp Brain Res 24:523–529
Hökfelt T, Lundberg JM, Terenius L, Jansco G, Kimmel J (1981) Avian pancreatic polypeptide (APP) immunoreactive neurons in the spinal cord and spinal trigeminal nucleus. Peptides 2:81–87
Hunt SP, Emson PC, Gilbert R, Goldstein M, Kimmel JR (1981) Presence of avian pancreatic polypeptide-like immunoreactivity in catecholamine and methionine enkephalin containing neurons within the central nervous system. Neurosci Lett 21:125–130
Kimmel JR, Hayden LJ, Pollock HG (1975) Isolation and characterization of a new pancreatic polypeptide hormone. J Biol Chem 250:9369–9376
Klein DC, Moore RY (1979) Pineal N-acetyltransferase and hydroxyindole-O-methyltransferase: Control by the retinohypothalamic tract and the suprachiasmatic nucleus. Brain Res 174:245–262
Lorén I, Alumets R, Håkanson R, Sundler F (1979) Immunoreactive pancreatic polypeptides (PP) occur in the central and peripheral nervous system: Preliminary immunocytochemical observations. Cell Tissue Res 200:179–186
Lundberg JM, Tatemoto K (1982) Pancreatic polypeptides and sympathetic vasoconstriction resistant to a adrenoceptor antagonists. Br J Pharmacol 77:335P
Lundberg JM, Hökfelt T, Anggard A, Kimmel J, Goldstein M, Markey K (1980) Coexistence of an avian pancreatic polypeptide (APP) immunoreactive substance and catecholamines in some peripheral and central neurons. Acta Physiol Scand 110:107–109
McDonald JK, Parnavelas JG, Karamanlikis AN, Brecha N (1982) The morphology and distribution of peptide-containing neurons in the adult and developing visual cortex of the rat. IV. Avian pancreatic polypeptide. J Neurocytol 11:985–995
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Moore RY (1983) Organization and function of a central nervous system circadian oscillator: the suprachiasmatic nucleus. Fed Proc 42:2783–2789
Moore RY, Marchand ER, Riley JN (1979) Suprachiasmatic nucleus afferents in the rat: An HRP-retrograde transport study. Neurosci Abstract 5:232
Price DA, Greenberg MJ (1977) Structure of a molluscan cardioexcitatory peptide. Science 197:670–671
Ribak CE, Peters A (1975) An autoradiographic study of the projections from the lateral geniculate body of the rat. Brain Res 92:241–268
Rossier J, Audigier V, Ling N, Cros J, Undenfriend S (1980) Metenkephalin-Arg6-Phe7, present in high amounts in brain of rat, cattle and man, is an opioid agonist. Nature 288:88–90
Rusak B (1977) Involvement of the primary optic tracts in mediation of light effects on hamster circadian rhythms. J Comp Physiol 118:165–172
Sternberger LA (1979) Immunocytochemistry (2nd Ed), Wiley, New York, pp 104–169
Swanson L, Cowan WM, Jones EG (1974) An autoradiographic study of the efferent connections of the ventral lateral geniculate nucleus in the albino rat and cat. J Comp Neurol 156:143–164
Tatemoto K (1982) Neuropeptide Y: Complete amino acid sequence of the brain peptide. Proc Natl Acad Sci USA 79:5485–5489
Tatemoto K, Calquist M, Mutt V (1982) Neuropeptide Y — a novel brain peptide with structural similarities to peptide YY and pancreatic peptide. Nature 268:659–660
Vincent SR, Skirboll L, Hökfelt T, Johansson O, Lundberg JM, Elde RP, Terenius L, Kimmel JR (1982) Coexistence of somatostatin and avian pancreatic polypeptide (APP)-like immunoreactivity in some forebrain neurons. Neuroscience 7:439–446
Weber E, Evans CJ, Samuelsson SJ, Brechas JD (1981) Novel peptide neuronal system in rat brain and pituitary. Science 214:1248–1251
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Moore, R.Y., Gustafson, E.L. & Card, J.P. Identical immunoreactivity of afferents to the rat suprachiasmatic nucleus with antisera against avian pancreatic polypeptide, molluscan cardioexcitatory peptide and neuropeptide Y. Cell Tissue Res. 236, 41–46 (1984). https://doi.org/10.1007/BF00216511
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DOI: https://doi.org/10.1007/BF00216511