Abstract
A polyclonal antiserum raised against crustacean cardioactive peptide labels 14 clusters of immunoreactive neurons in the protocerebrum of the spiders Tegenaria atrica and Nephila clavipes, and the harvestman (opilionid) Rilaena triangularis. In all species, these clusters possess the same number of neurons, and share similar structural and topological characteristics. Two sets of bilateral symmetrical neurons associated with the optic lobes and the arachnid “central body” were analysed in detail, comparing the harvestman R. triangularis and the spiders Brachypelma albopilosa (Theraphosidae), Cupiennius salei (Lycosidae), Tegenaria atrica (Agelenidae), Meta segmentata (Metidae) and Nephila clavipes (Araneidae). Sixteen neurons have been identified that display markedly similar axonal pathways and arborization patterns in all species. These neurons are considered homologues in the opilionid and the araneid brains. We presume that these putative phylogenetically persisting neurons represent part of the general morphological pattern of the arachmid brain.
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References
Anderson DT (1973) Embryology and phylogeny in annelids and arthropds Pergamon Press, Oxford
Arbas EA, Meinertzhagen IA, Shaw SR (1991) Evolution in nervous systems. Annu Rev Neurosci 14:9–38
Audehm U, Trube A, Dircksen H (1993) Patterns and projections of crustacean-cardioactive-peptide-immunoreactive neurons of the terminal ganglion of crayfish. Cell Tissue Res 272:473–485
Babu KS (1985) Patterns of arrangement and connectivity in the central nervous system of arachnids. In: Barth FG (ed) Neurobiology of arachnids. Springer, Berlin Heidelberg New York, pp 3–19
Bergström J (1979) Morphology of fossil arthropods as a guide to phylogenetic relationships (1979) In: Gupta AP (ed) Arthropod phylogeny. Van Norstrand, New York, pp 3–56
Breidbach O (1990) Constant topological organization of the coleopteran metamorphosing nervous system — analysis of persistent elements in the nervous system of Tenebrio molitor. J Neurobiol 21:990–1001
Breidbach O (1992) Ist das Arthropoden-Hirn zweimal entstanden? Natur Museum 122:301–310
Breidbach O (1994) Common “bauplan” of the arthropod nervous systems? In: Breidbach O, Kutsch W (eds) The nervous systems of invertebrates — an evolutionary and comparative approach. Birkhäuser, Basel (in press)
Breidbach O, Dircksen H (1991) Crustacean cardioactive peptide-immunoreactive neurons in the ventral nerve cord and the brain of the meal beetle Tenebrio molitor during postembryonic development. Cell Tissue Res 265:129–144
Breidbach O, Kutsch W (1990) Structural homology of identified motoneurons in larval and adult stages of hemi- and holometabolous insects. J Comp Neurol 297:392–409
Breidbach O, Wegerhoff R (1993) Neuroanatomy of the central nervous system of the harvestman, Rilaena triangularis (HERBST 1799) (Arachnida; Opiliones)—principal organization, GABA-like and serotonin-immunohistochemistry. Zool Anz 230:55–81
Breidbach O, Wegerhoff R (1994) FMRFamide-like immunoreactive neurons in the brain of the beetle, Tenebrio molitor (Coleoptera: Tenebrionidae): Constancies and variations in development from the embryo to the adult. Int J Insect Morphol Embryol 29:383–404
Bullock TH, Horridge GA (1965) Structure and function in the nervous systems of invertebrates, vol 2. Freeman, San Francisco
Cheung CC, Loi PK, Sylwester AB, Lee TD, Tublitz NJ (1992) Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta. FEBS Lett 313:165–168
Dircksen H (1994) Distribution and physiology of crustacean cardioactive peptide in arthropods. Can J Zool 71 (in press)
Dircksen H, Keller R (1988) Immunocytochemical localization of CCAP, a novel crustacean cardioactive peptide in the nervous system of the shore crab, Carcinus maenas L. Cell Tissue Res 256:347–360
Dircksen H, Müller A, Keller R (1991) Crustacean cardioactive peptide in the nervous system of the locust Locusta migratoria: an immunocytochemical study of the ventral nerve cord and peripheral innervation. Cell Tissue Res 263:439–457
Foelix RF (1992) Biologie der Spinnen. Thieme, Stuttgart
Groome JR, Townley MA, De Tschaschell M, Tillinghast EK (1991) Detection and isolation of proctolin-like immunoreactivity in arachnids: possible cardioregulatory role for proctolin in the orb-weaving spiders Argiope and Araneus. J Insect Physiol 37:9–19
Gupta AP (1987) Evolutionary trends in the central and mushroom bodies of the arthropod brain. A dilemma. In: Gupta AP (ed) Arthropod brain. Wiley, New York, pp 27–44
Hammen L van der (1989) An introduction in comparative arachnology. SPB Academic Press, Den Haag
Hanström B (1919) Zur Kenntnis des zentralen Nervensystems der Arachnoiden und Pantopoden. PhD thesis, Lund AB Skanska
Hanström B (1921) Über die Histologie und vergleichende Anatomie der Sehganglien und der Globuli der Araneen. Kungl Svenska Vetensk Akad Hand 61:1–39
Hanström B (1923) Further notes on the central nervous system of arachnids, scorpions, phalangids and trap-door spiders. J Comp Neurol 35:249–272
Hanström B (1926) Untersuchungen über die relative Größe der Gehirnzentren verschiedener Arthropoden unter Berücksichtigung der Lebensweise. Z Mikrosk Anat Forsch 7:135–190
Hanström B (1928) Vergleichende Anatomie des Nervensystems der wirbellosen Tiere. Springer, Berlin
Hanström B (1935) Fortgesetzte Untersuchungen über das Araneengehirn. Zool Jb Anat 59:455–478
Holmgren E (1916) Zur vergleichenden Anatomie des Gehirns der Polychaeten, Onychophora, Xiphosuren, Arachniden, Crustaceen, Myriapoden und Insekten. Kungl Svensk Vetensk Handl 56:1–303
Juberthie C (1983) Neurosecretory systems and neurohemal organs of terrestrial Chelicerata (Arachnida). In: Gupta AP (ed) Neurohemal organs of arthropods. Thomas, Springfield, Illinois, pp 149–203
Kutsch W, Breidbach O (1994) Homologous structures in the nervous systems of Arthropoda. Adv Insect Physiol 24:1–113
Lehman HK, Murgiuc CM, Miller TA, Lee TD, Hildebrand JG (1993) Crustacean cardioactive peptide in the sphinx moth, Manduca sexta. Peptides 14:735–741
Meyer W, Schlesinger C, Poehling HM, Ruge W (1984) Comparative quantitative aspects of putative neurotransmitters in the central nervous system of spiders (Arachnida: Araneida). Comp Biochem Physiol [C] 78:357–362
Nentwig W (1987) Ecophysiology of spiders. Springer, Berlin Heidelberg New York, pp 380–388
Remane A (1956) Die Grundlagen des natürlichen Systems, der vergleichenden Anatomie und der Phylogenetik. Akad Verl Geest & Portig, Leipzig
Schmid A, Duncker M (1993) Histamine immunoreactivity in the central nervous system of the spider Cupiennius salei. Cell Tissue Res 273:533–545
Schmid A, Duncker M, Spörhase-Eichmann U (1990) Verteilung FMRFamid- und GABA-artiger Immunoreaktivität im ZNS der Jagdspinne Cupiennius salei. Verh Dtsch Zool Ges 83:639–640
Seyfarth EA, Hammer K, Grünert U (1990) Serotonin-like immunoreactivity in the CNS of spiders. In: Elsner N, Roth G (eds) Brain-perception-cognition. Thieme, Stuttgart, p 331
Seyfarth E, Hammer K, Spörhase-Eichmann U, Hörner M, Vullings HGB (1993) Octopamine immunoreactive neurons in the fused central nervous system of spiders. Brain Res 611:193–206
Stangier J, Keller R (1990) Occurrence of the crustacean cardioactive peptide (CCAP) in the nervous system of the crayfish, Orconectus limosus. In: Wiese K, Krenz WD, Tautz J, Reichert H, Mulloney B (eds) Frontiers in crustacean neurobiology. Birkhäuser, Basel, pp 394–400
Stangier J, Hilbich C, Beyreuther K, Keller R (1987) Unusual cardioactive peptide (CCAP) from pericardial organs of the shore crab Carcinus maenas. Proc Natl Acad Sci USA 84:575–579
Stangier J, Hilbich C, Keller R (1989) Occurrence of crustacean cardioactive peptide (CCAP) in the nervous system of an insect, Locusta migranoria. J Comp Physiol [B] 159:5–11
Sternberger A (1979) The unlabeled antibody peroxidase-antiper-oxidase (PAP) method. Wiley, New York, pp 104–169
Strausfeld NJ, Barth FG (1993) Two visual systems in one brain: neuropils serving the secondary eyes of the spider Cupiennius salei. J Comp Neurol 328:43–62
Strausfeld NJ, Weltzien P, Barth FG (1993) Two visual systems in one brain: neuropils serving the principal eyes of the spider Cupiennius salei. J Comp Neurol 328:43–62
Trube A, Audehm U, Dircksen H (1994) Crustacean cardioactive peptide-immunoreactive neurons in the ventral nervous system of crayfish. J Comp Neurol (in press)
Wegerhoff R, Breidbach O (1994) Comparative aspects of the Chelicerata nervous system. In: Breidbach O, Kutsch W (eds) The nervous systems of invertebrates — an evolutionary and comparative approach. Birkhäuser, Basel (in press)
Weltzien P (1988) Vergleichende Neuroanatomie des Spinnenhirns unter besonderer Berücksichtigung des Zentralkörpers. PhD thesis. University of Frankfurt, Germany
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Breidbach, O., Dircksen, H. & Wegerhoff, R. Common general morphological pattern of peptidergic neurons in the arachnid brain: crustacean cardioactive peptide-immunoreactive neurons in the protocerebrum of seven arachnid species. Cell Tissue Res 279, 183–197 (1995). https://doi.org/10.1007/BF00300703
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DOI: https://doi.org/10.1007/BF00300703