Summary
The location of the somata, course of the main tracts, and fiber distribution of the serotonin neurons in the turtle brain were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical method with antibodies against serotonin (5-hydroxytryptamine). The somata of the serotonin neurons were distributed in the reticular formation of the brainstem from the mesencephalon to the lower medulla level and in a resticted region of the hypothalamus, viz. the paraventricular organ (PVO). In the PVO the serotonin neurons were seen to have the appearance of cerebrospinal fluid-contacting neurons. Analysis of serial sections cut in the frontal and sagittal planes revealed a widespread distribution of the serotonin immunoreactive fibers in the turtle brain. Prominent concentrations of the serotonin immunoreactive fibers were found in the lateral portion of the striatum, the ventral portion of the septum, the nucleus corporis geniculati lateralis, the nucleus pretectalis, the nucleus isthmi parvocellularis, the optic tectum, and the lateral edge of the reticular formation of the brainstem. Ascending and descending serotonin pathways could be defined: the ascending pathway originated mainly from the nucleus profundus mesencephali caudalis, nucleus lemnisci lateralis, nucleus reticularis isthmi and, less prominently, from the nucleus raphe superior pars lateralis, and the descending pathway arose predominantly from the nucleus raphe inferior. The fibers of the ascending pathway projected widely in the prosencephalon and mesencephalon, via the medial forebrain bundle. The descending pathway ran through the ventral and lateral portion of the medulla oblongata and spinal cord.
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References
Baraban CD, Ulinski PS (1981) Organization of thalamic afferents to anterior dorsal ventricular ridge in turtles. 1. Projections of thalamic nuclei. J Comp Neurol 200:95–129
Bass AH, Northcut RG (1981) Retinal recipient nuclei in the painted turtle, Chrysemys picta: An autoradiographic and HRP study. J Comp Neurol 199:97–119
Baumgarten HG, Braak H (1967) Catecholamine im Hypothalamus vom Goldfish (Carassius auratus). Z Zellforsch 80:246–263
Braak H, Baumgarten HG, Falck B (1968) 5-Hydroxytryptamin im Gehirn der Eidechse (Lacerta viridis und Lacerta muralis). Z Zellforsch 90:161–185
Cruce WLR, Nieuwenhuys R (1974) The cell masses in the brain stem of the turtle Testude hermanni; A topographical and topological analysis. J Comp Neurol 156:277–306
Fuxe K, Ljunggren L (1965) Cellular localization of monoamines in the upper brain stem of the pigeon. J Comp Neurol 125:355–382
Fuxe K, Hökfelt T, Ungerstedt U (1968) Localization of indolealkylamines in CNS. In: Garattini S, Shore PA (eds) in Adances in Pharmacology. Vol 6 Academic Press New York
Hall WC, Ebner FF (1970) Paralleles in the visual afferent projections of the thalamus in the hedgehog (Paraechinus hypomelas) and the turtle (Pseudemys scripta). Brain Behav Evol 3:135–154
Huber GC, Crosby EC (1933) The reptilian optic tectum. J Comp Neurol 57:57–163
Johnston JB (1915) The cell masses in the forebrain of the turtle, Cistude carolina. J Comp Neurol 25:393–468
Kappers ACU, Huber C, Crosby EC (1973) The comparative anatomy of the nervous system of vertebrates including man. Vol. 1, 11 and 111 Macmillan New York
Kojima M, Takeuchi Y, Goto M, Sano Y (1982) Immunohistochemical study on the distribution of serotonin fibers in the spinal cord of the dog. Cell Tissue Res 226:477–491
Kojima M, Takeuchi Y, Goto M, Sano Y (1983a) Immunohistochemical study on the localization of serotonin fibers and terminals in the spinal cord of the monkey (Macaca fuscata). Cell Tissue Res 229:23–36
Kojima M, Takeuchi Y, Goto M, Sano Y (1983b) Immunohistochemical study on the distribution of serotonin-containing cell bodies in the brain stem of the dog. Acta Anat 115:8–22
Lidov HWG, Molliver ME (1982) An immunohistochemical study of serotonin neuron development in the rat: Ascending pathways and terminal fields. Brain Res Bull 8:389–430
Lidov HGW, Grzanna R, Molliver ME (1980) The serotonin innervation of the cerebral cortex in the rat — an immunohistochemical analysis. Neuroscience 5:207–227
Marschall C (1980) Hypothalamic monoamines in lizards (Lacerta). A histofluorescence study. Cell Tissue Res 205:95–105
Moore RY, Heller A (1967) Monoamine levels and neuronal degeneration in the rat brain following lateral hypothalamic lesion. J Pharmacol Exper Ther 156:12–33
Parent A (1973a) Demonstration of a catecholaminergic pathway from the midbrain to the strio-amygdaloid complex in the turtle (Chrysemys picata). J Anat 114:370–387
Parent A (1973b) Distribution of monoamine-containing nerve terminals in the brain of the painted turtle, Chrysemys picta. J Comp Neurol 148:153–166
Parent A (1976) Strial afferent connection in the turtle (Chrysemys picta) as revealed by retrograde axonal transport of horseradish peroxidase. Brain Res 108:25–36
Parent A (1981) The anatomy of serotonin-containing neurons across phylogeny. In: Jacobs BL, Gelperin A (eds) Serotonin neurotransmission and behavior. The MIP Press, England
Parent A, Poirier LJ (1971) Occurrence and distribution monoamine-containing neurons in the brain of the painted turtle, Chrysemys picta. J Anat 110:81–89
Parent A, Poitras D (1974) The origin and distribution of catecholaminergic axon terminals in the cerebral cortex of the turtle (Chrysemys picta). Brain Res 78:345–358
Parent A, Poitras D (1974) Morphological organization of monoamine-containing neurons in the hypothalamus of the painted turtle (Chrysemys picta). J Comp Neurol 154:379–394
Parent A, Descarries L, Beaudet A (1981) Organization of ascending serotonin systems in the adults rat brain. A radioautographic study after intraventricular administration of [3H] 5-Hydroxytryptamine. Neuroscience 6:115–138
Ramón P (1896) Estructural del encéfalo der cameleón. Revista trimestral micrográfica Vol 1. pp 46–82
Sano Y, Takeuchi Y, Kimura H, Goto M, Kawata M, Kojima M, Matsuura T, Ueda S, Yamada H (1982) Immunohistochemical studies on the processes of serotonin neurons and their ramification in the central nervous system — with regard to the possibility of the existance of GOLGI's rete nervosa diffusa. Arch Histol Jpn 45:305–316
Sano Y, Ueda S, Yamada H, Takeuchi Y, Goto M, Kawata M (1983) Immunohistochemical demonstration of serotonin-containing CSF-contacting neurons in the submammalian paraventricular organ. Histochemistry 77:423–430
Steinbusch HWM (1981) Distribution of serotonin-immunoreactivity in the central nervous system of the rat — Cell bodies and terminals. Neuroscience 6:557–618
Steinbusch HWM, Verhofstad AA, Joosten HWJ (1978) Localization of serotonin in the central nervous system by immunohistochemistry: Description of a specific and sensitive technique and some applications. Neuroscience 3:811–819
Steinbusch HWM, Verhofstad AAJ, Penke B, Varga J, Joosten HWJ (1981) Immunohistochemical characterization of monoamine-containing neurons in the central nervous system by antibodies to serotonin and noradrenaline. A study in the rat and lamprey (Lampetra fluviatilia). Acta Histochem Suppl XXIV:107–122
Takeuchi Y, Kimura H, Sano Y (1982a) Immunohistochemical demonstration of the distribution of serotonin neurons in the brainstem of the rat and cat. Cell Tissue Res 224:247–267
Takeuchi Y, Kimura H, Matuura T, Sano Y (1982b) Immunohistochemical demonstration of the organization of serotonin neurons in the brain of the monkey (Macaca fuscata). Acta Anat 114:106–124
Takeuchi Y, Kimura H, Matuura T, Yonezawa T, Sano Y (1983) Distribution of serotonergic neurons in the central nervous system: a peroxidase-antiperoxidase study with anti-serotonin antibodies. J Histochem Cytochem 31:181–185
Yamada H, Takeuchi Y, Sano Y (1983) Immunohistochemical studies on the serotonin neuron system in the brain of the chicken (Gallus domesticus). I. The distribution of the neuronal somata. Biogenic Amines 1: (in press)
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Supported by a grant from Ministry of Education, Science and Culture of Japan (No. 57214028)
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Ueda, S., Takeuchi, Y. & Sano, Y. Immunohistochemical demonstration of serotonin neurons in the central nervous system of the turtle (Clemmys japonica). Anat Embryol 168, 1–19 (1983). https://doi.org/10.1007/BF00305395
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DOI: https://doi.org/10.1007/BF00305395