Summary
Neurons displaying somatostatin or vasoactive intestinal polypeptide (VIP) immunoreactivity were detected among the amacrine cells in the retina of baboon, cynomolgus monkey, squirrel monkey, cow, pig, cat, rabbit, guinea-pig, rat, mouse, frog and goldfish. Generally, immunoreactive cell bodies were located in the inner nuclear layer with processes ramifying in three more or less well-defined sublayers in the inner plexiform layer. The density of the sublayers and their location varied with the peptide and species investigated. In most cases there was a sublayer in the outermost part (Ramon y Cajal's sublamina 1) of the inner plexiform layer and this sublayer was usually the best developed. In some species a few somatostatin fibres were also detected in the outer plexiform layer, suggesting that some interplexiform cells contain somatostatin. In the baboon VIP was found exclusively in interstitial amacrine cells which have their cell bodies and processes entirely within the inner plexiform layer.
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Alumets J, Håkanson R, Lundqvist G, Sundler F, Thorell J (1980) Ontogeny and ultrastructure of somatostatin and calcitonin cells in the thyroid gland of the rat. Cell Tissue Res 206:193–201
Brecha N, Hendrickson A, Florén I, Karten HJ (1982) Localization of substance P-like immunoreactivity within the monkey retina. Invest Ophthalmol Vis Sci 23:147–153
Brecha N, Karten HJ, Laverack C (1979) Enkephalin-containing amacrine cells in the avian retina: Immunohistochemical localization. Proc Natl Acad Sci USA 76:3010–3014
Brecha N, Karten HJ, Schenker C (1981) Neurotensin-like and somatostatin-like immunoreactivity within amacrine cells of the retina. Neuroscience 6:1329–1340
Buckerfield M, Oliver J, Chubb IW, Morgan IG (1981) Somatostatin-like immunoreactivity in amacrine cells of the chicken retina. Neuroscience 6:689–695
Cajal SRY (1973) In: Rodieck RW (ed) The vertebrate retina. Principles of structure and function. WH Freeman, San Francisco, pp 777–904
Coons AH, Leduc EH, Connolly JM (1955) Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med 102:49–60
Costa M, Buffa R, Furness JB, Solcia E (1980) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65:157–165
Ehinger B (1982) Retinal neurotransmitter substances. Retina (in press)
Eriksen EF, Larsson L-I (1981) Neuropeptides in the retina: Evidence for differential topographical localization. Peptides 2:153–157
Eskay RL, Long RT, Iuvone PM (1980) Evidence that TRH, somatostatin and substance P are present in neurosecretory elements of the vertebrate retina. Brain Res 196:554–559
Fukuda M, Kuwayama Y, Shiosaka S, Ishimoto I, Shimizu Y, Takagi H, Inagaki S, Sakanaka M, Semba E, Takatsuki K, Tohyama M (1981) Demonstration of a substance P-like immunoreactivity in retinal cells of the rat. Neurosci Lett 23:239–242
Humbert J, Pradelles P, Gros C, Dray F (1979) Enkephalin-like products in embryonic chicken retina. Neurosci Lett 12:259–263
Håkanson R, Leander S, Sundler F, Uddman R (1981) P-type nerves: Purinergic or peptidergic? In: Grossman MI, Brazier MAB, Lechago J (eds) Cellular basis of chemical messengers in the digestive system. Academic Press, New York, pp 169–199
Hökfelt T, Johansson O, Ljungdahl Å, Lundberg JM, Schultzberg M (1980) Peptidergic neurones. Nature 284:515–521
Johansson O, Hökfelt T, Pernow B, Jeffcoate SL, White N, Steinbusch HWM, Verhofstad AAJ, Emson PC, Spindel E (1981) Immunohistochemical support for three putative transmitters in one neuron: Coexistence of 5-hydroxytryptamine, substance P- and thyrotropin releasing hormone-like immunoreactivity in medullary neurons projecting to the spinal cord. Neuroscience 6:1857–1881
Karten HJ, Brecha N (1980) Localization of substance P immunoreactivity in amacrine cells of the retina. Nature 283:87–88
Karten HJ, Brecha N (1982) Neuropeptides in the vertebrate retina. In: Bradford HF (ed) Neurotransmitter interaction and compartmentation. Plenum Press, New York London, (in press)
Krisch B, Leonhardt H (1979) Demonstration of a somatostatin-like activity in retinal cells of the rat. Cell Tissue Res 204:127–140
Lake N, Patel YC (1980) Neurotoxic agents reduce retinal somatostatin. Brain Res 181:234–236
Longshore MA, Makman MH (1981) Stimulation of retinal adenylate cyclase by vasoactive intestinal peptide (VIP). Eur J Pharmacol 70:237–240
Lorén I, Tornqvist K, Alumets J (1980) VIP (Vasoactive intestinal polypeptide)-immunoreactive neurons in the retina of the rat. Cell Tissue Res 210:167–170
Lundqvist I, Sundler F, Ahrén B, Alumets J, Håkanson R (1979) Somatostatin, pancreatic polypeptide, substance P and neurotensin: cellular distribution and effects on stimulated insulin secretion in the mouse. Endocrinology 104:832–838
Osborne NN, Nicholas DA, Cuello AC, Dockray GJ (1981) Localization of cholecystokinin immunoreactivity in amacrine cells of the retina. Neurosci Lett 26:31–35
Rorstad OP, Brownstein M, Martin JB (1979) Immunoreactive and biologically active somatostatin-like material in rat retina. Proc Natl Acad Sci USA:3019–3023
Rorstad OP, Senterman MK, Hoyte KM, Martin JB (1980) Immunoreactive and biologically active somatostatin-like material in the human retina. Brain Res 199:488–492
Schorderet M, Sovilla J-Y, Magistretti PJ (1981) VIP- and glucagon-induced formation of cyclic AMP in intact retina in vitro. Eur J Pharmacology 71:131–133
Shapiro B, Kronheim S, Pimstone B (1979) The presence of immunoreactive somatostatin in rat retina. Horm Metab Res 11:79–80
Sporrong B, Falmer S, Robboy SJ, Alumets J, Håkanson R, Ljungberg O, Sundler F (1982) Neurohormonal peptides in ovarian carcinoids. An immunohistochemical study of 81 primary carcinoids and of intraovarian metastases from six mid-gut carcinoids. Cancer 49:68–74
Sternberger LA (1979) The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Immunocytochemistry. Wiley and Sons, New York, pp 104–169
Tornqvist K, Lorén I, Håkanson R, Sundler F (1981) Peptide-containing neurons in the chicken retina. Exp Eye Res 33:55–64
Uddman R, Kitajiri M, Sundler F (1982) The autonomic innervation of the middle ear. Ann Otol Rhinol Laryngol (in press)
Unger WG, Butler JM, Cole DF, Bloom SR, MacGregor GP (1981) Substance P, vasoactive intestinal polypeptide (VIP) and somatostatin levels in ocular tissue of normal and sensorily denervated rabbit eyes. Exp Eye Res 32:797–801
Watling KJ, Dowling JE (1981) Vasoactive intestinal peptide stimulates cAMP accumulation in the carp retina. Invest Ophthalmol Vis Sci 20: No 3, Suppl 167
Yamada T, Marshak D, Basinger S, Walsh J, Morley J, Stell W (1980) Somatostatin-like immunoreactivity in the retina. Proc Natl Acad Sci USA 77:1691–1695
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Tornqvist, K., Uddman, R., Sundler, F. et al. Somatostatin and VIP neurons in the retina of different species. Histochemistry 76, 137–152 (1982). https://doi.org/10.1007/BF00501917
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DOI: https://doi.org/10.1007/BF00501917