Summary
A retrovirus which encodes β-galactosidase was used to infect embryonic rat striatal cells before grafting these cells into the lesioned adult rat striatum. Examination of the grafts after long term survival (8 months) revealed that a few small and large cells expressed large amounts of bacterial β-galactosidase activity. The larger diameter cells were identified as neurones by their size, shape and presence of neuronal processes. The identity of the small diameter cell types was not established.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Björklund A, Stenevi U, Schmidt R, Dunnett SB, Cage FH (1983) Intracerebral grafting of neuronal cell suspensions. Acta Physiol Scand Suppl 522:1–9
Gage FH, Wolff JA, Rosenberg MB, Xu L, Yee J-K, Shults C, Friedmann T (1987) Grafting genetically modified cells to the brain: possibilities for the future. Neuroscience 23:795–807
Gray GE, Glover JC, Majors J, Sanes JR (1988) Radial arrangements of clonally related cells in the chicken optic tectum: lineage analysis with a recombinant retrovirus. Proc Natl Acad Sci 85:7356–7360
Huzzar D, Balling R, Kothary R, Magli MC, Hozumi N, Rossant J, Bersntein A (1985) Insertion of a bacterial gene into the mouse germ line using an infectious retrovirus vector. Proc Natl Acad Sci USA 82:8587–8591
Iscason O, Dawbarn D, Brundin P, Gage FH, Emson PC, Björklund A, (1987) Neural grafting in a rat model of Huntington's disease: striosomal-like organisation of striatal grafts as revealed by acetylcholinesterase histochemistry, immunocytochemistry and receptor autoradiography. Neuroscience 22:481–497
Luskin MB, Pearlman AL, Sanes JR (1988) Cell lineage in the cerebral cortex of the mouse studied in vivo and in vitro with a recombinant retrovirus. Neuron 1:635–647
Mann R, Mulligan RC, Baltimore D (1983) Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 35:153–159
Mulligan RC (1983) Construction of highly transmissible mammalian cloning vehicles derived from murine retroviruses. In: Inouye M (eds) Experimental manipulation of gene experession. Academic Press, London New York, pp 155–1973
Price J (1987) Retroviruses and the study of cell lineage. Dev 101:409–419
Price J, Turner D, Cepko C (1987) Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc Natl Acad Sci USA 84:156–160
Price J, Thurlow L (1988) Cell lineage in the rat cerebral cortex: a study using retrovirual mediated gene transfer. Development 104:473–482
Sanes JR (1989) Analysing cell lineage with a recombinant retrovirus. Trends Neurosci 12:21–27
Sanes JR, Rukenstein JLR, Nicolas J-F (1986) Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos. EMBO J 5:3133–3142
Shimohama S, Rosenberg MB, Fagan AM, Wolff JA, Short MP, Breakfield XO, Friedmann T, Gage FH (1989) Grafting genetically modified cells into the rat brain: characteristics of E. coli β-galactosidase as a report gene. Mol Brain Res 5:271–278
Turner DL, Cepko CL (1987) A common progenitor for neurons and glia persists in rat retina late in development. Nature 328:131–136
Walsh C, Cepko C (1988) Clonally related cortical cells show several migration patterns. Science 241:1342–1345
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Emson, P.C., Shoham, S., Feler, C. et al. The use of a retroviral vector to identify foetal striatal neurones transplanted into the adult striatum. Exp Brain Res 79, 427–435 (1990). https://doi.org/10.1007/BF00608254
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00608254