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Localization of arginine kinase in muscle fibres ofDrosophila melanogaster

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Summary

Arginine kinase (AK), a monomeric protein of mol. wt. 40 000, was purified from adultDrosophila melanogaster. Antiserum to AK was raised in rabbits; its specificity was established by immunodiffusion tests and by immunoreplicas of electropherograms. After electrophoresis in non-denaturing conditions, the immunoreactive material was shown to possess enzymatic activity. Localization of AK by indirect immunofluorescence on washed myofibrils showed that at least a fraction of the total cellular AK is bound to the contractile apparatus. In muscle fibres of third instar larvae and in isolated adult fibrillar flight muscle myofibrils, AK was localized within the Z-line region. Adult tubular muscle fibres (tergal depressor of the trochanter) showed fluorescence in the A-band. In all cases, the localization was independent of the state of contraction. Vertebrate B- and M-type creatine kinases have previously been localized at the Z-line and A-band (M-line) regions, respectively. Isoenzymic forms of AK have not been detected inDrosophila, however.

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  1. Institute for Cell Biology, Swiss Federal Institute of Technology, Hönggerberg, CH - 8093, Zürich, Switzerland

    Alois B. Lang, Christoph Wyss & Hans M. Eppenberger

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  1. Alois B. Lang
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  2. Christoph Wyss
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Lang, A.B., Wyss, C. & Eppenberger, H.M. Localization of arginine kinase in muscle fibres ofDrosophila melanogaster . J Muscle Res Cell Motil 1, 147–161 (1980). https://doi.org/10.1007/BF00711796

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