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Retrograde transport of D-[3H]-aspartate injected into the monkey amygdaloid complex

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Summary

The possibility that certain of the afferents of the primate amygdaloid complex use an excitatory amino acid transmitter was evaluated by injecting D-[3H]-aspartate into the amygdala of twoMacaca fascicularis monkeys. The distribution of D-[3H]-aspartate labeled neurons was compared with those labeled with the nonselective retrograde tracer WGA-HRP injected at the same location as the isotope. Retrogradely labeled cells of both types were observed in a variety of cortical and subcortical structures and in discrete regions within the amygdala. D-[3H]-aspartate labeled neurons were observed in layers III and V of the frontal, cingulate, insular and temporal cortices. In the hippocampal formation, heavily labeled cells were observed in the CA1 region and in the deep layers of the entorhinal cortex. Of the subcortical afferents, the claustrum and the midbrain peripeduncular nucleus contained the greatest number of D-[3H]-aspartate labeled cells. Subcortical afferents that are not thought to use excitatory amino acids, such as the cholinergic neurons of the basal nucleus of Meynert, did not retrogradely transport the isotope. Within the amygdala, the most conspicuous labeling was in the paralaminar nucleus which forms the rostral and ventral limits of the amygdala. When the D-[3H]-aspartate injection involved the basal nucleus, many labeled cells were also observed in the lateral nucleus. Retrograde transport of D-[3H]-aspartate injected into the amygdala, therefore, appears to demonstrate a subpopulation of inputs that may use an excitatory amino acid transmitter.

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Abbreviations

36pl Area:

36 (lateral portion of polar region)

36pm Area:

36 (medial portion of polar region)

36r Area:

36 (rostral portion)

A1:

Primary auditory field

ABmg:

Accessory basal nucleus of the amygdala (magnocellular portion)

ABpc:

Accessory basal nucleus of the amygdala (parvicellular portion)

ac:

Anterior commissure

amts:

Anterior middle temporal sulcus

as:

Arcuate sulcus

B:

Basal nucleus of the amygdala

BNM:

Basal nucleus of Meynert

CA1:

Field CA1 of the Hippocampus

cc:

Corpus callosum

C:

Central nucleus of the amygdala

CD:

Caudate nucleus

Cl:

Claustrum

cs:

Cingulate sulcus

EC:

Entorhinal cortex

G:

Gustatory cortex

H:

Hippocampus

I:

Intercalated nucleus of the amygdala

Ia:

Agranular insular cortex

Id:

Dysgranular insular cortex

Ig:

Granular insular cortex

ils:

Inferior limiting sulcus

LA:

Lateral auditory field

LGN:

Lateral geniculate nucleus

L:

Lateral nucleus of the amygdala

los:

Lateral orbital sulcus

mos:

Medial orbital sulcus

OT:

Olfactory tubercle

ot:

Optic tract

ots:

Occipitotemporal sulcus

PAC:

Periamygdaloid cortex

PA:

Posterior auditory field

Pi:

Parainsular cortex

PIR:

Piriform cortex

PL:

Paralaminar nucleus of the amygdala

pmts:

Posterior middle temporal sulcus

PPN:

Peripeduncular nucleus

ps:

Principal sulcus

RA:

Rostral auditory field

RI:

Retroinsular cortex

ros:

Rostral sulcus

rs:

Rhinal sulcus

sls:

Superior limiting sulcus

SN:

Substantia nigra

sts:

Superior temporal sulcus

TA Area:

TA of Von Bonin and Bailey

TE Area:

TE of Von Bonin and Bailey

TEO Area:

TEO of Von Bonin and Bailey

TF Area:

TF of Von Bonin and Bailey

TH Area:

TH of Von Bonin and Bailey

V:

Lateral ventricle

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Authors and Affiliations

  1. The Salk Institute, P.O. Box 85800, 92186-5800, San Diego, CA, USA

    David G. Amaral

  2. Department of Anatomy, University of Navarra, Apdo. 273, E-31080, Pamplona, Spain

    Ricardo Insausti

Authors
  1. David G. Amaral
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  2. Ricardo Insausti
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Amaral, D.G., Insausti, R. Retrograde transport of D-[3H]-aspartate injected into the monkey amygdaloid complex. Exp Brain Res 88, 375–388 (1992). https://doi.org/10.1007/BF02259113

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  • DOI: https://doi.org/10.1007/BF02259113

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