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Control of IsAHP in mouse hippocampus CA1 pyramidal neurons by RyR3-mediated calcium-induced calcium release

  • Cellular Neurophysiology
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Abstract

In several neuronal preparations, the ryanodine-sensitive calcium store was reported to participate in the generation of slow afterhyperpolarization currents (IsAHP) involved in spike frequency adaptation. We show that calcium release from the ryanodine-sensitive calcium store is a major determinant of the triggering of IsAHP in mouse CA1 pyramidal neurons. Whole-cell patch clamp recordings in hippocampus slices show that the intracellular calcium stores depletion using an inhibitor of the endoplasmic reticulum Ca2+-ATPase (5 μM cyclopiazonic acid), as well as the specific blockade of ryanodine receptors (100 μM ryanodine) both reduced the IsAHP by about 70%. Immunohistology, using an anti-RyR3 specific antibody, indicates that RyR3 expression is particularly enriched in the CA1 apical dendrites (considered as the most important site for sAHP generation). We show that our anti-RyR3 antibody acts as a functional RyR3 antagonist and induced a reduction in IsAHP by about 70%. The additional ryanodine application (100 μ M) did not further affect IsAHP, thus excluding RyR2 in IsAHP activation. Our results argue in favor of a specialized function of RyR3 in CA1 pyramidal cells in triggering IsAHP due to their localization in the apical dendrite.

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Acknowledgment

P.C. was supported by fellowships from the Ministère de l’Education Nationale, de la Recherche Scientifique et de la Technologie and from the Association Française contre les Myopathies. This work was supported by a grant from the Association Française contre les Myopathies. We gratefully acknowledge Dr. Nicolas Morel and Dr. Jean Pierre Denizot (CNRS, Gif/Yvette, France) for their precious expertise in biochemistry and immunohistology.

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  1. Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Avenue de la Terrasse, 91198, Gif sur Yvette, France

    Y. van de Vrede, P. Fossier, G. Baux & P. Chameau

  2. Swammerdam Institute for Life Sciences, Center for NeuroSciences, University of Amsterdam, Kruislaan 320, 1098 SM, Amsterdam, The Netherlands

    M. Joels & P. Chameau

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  1. Y. van de Vrede
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Correspondence to P. Chameau.

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van de Vrede, Y., Fossier, P., Baux, G. et al. Control of IsAHP in mouse hippocampus CA1 pyramidal neurons by RyR3-mediated calcium-induced calcium release. Pflugers Arch - Eur J Physiol 455, 297–308 (2007). https://doi.org/10.1007/s00424-007-0277-4

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