A method for measuring both glutamine and glutamate levels and stable isotopic enrichments☆
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Growth hormone enhances fat-free mass and glutamine availability in patients with short-bowel syndrome: An ancillary double-blind, randomized crossover study
2014, American Journal of Clinical NutritionA novel method for simultaneous measurement of concentration and enrichment of NO synthesis-specific amino acids in human plasma using stable isotopes and LC/MS ion trap analysis
2014, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :In order to develop new nutritional strategies, the effect of the administration of specific supplements on the glutamine–citrulline–arginine pathway should be determined with stable isotope studies. To measure isotope enrichments of amino acid tracers in plasma samples, gas chromatography mass spectrometry (GC/MS) [13–15], gas chromatography isotope ratio mass spectrometry (GC/C/IRMS) [16–20], liquid chromatography mass spectrometry (LC/MS) [21], and liquid chromatography isotope ratio mass spectrometry (LC/IRMS) [22–24] have been used. Although the isotope-ratio mass spectrometry (IRMS) techniques are superior for precise isotopic measurement compared with the MS techniques, the IRMS techniques have the disadvantages of being more expensive, being less sensitive, isotope dedicated and requiring an optimal separation of the compounds of interest and other co-eluting peaks for correct measurement.
<sup>13</sup>C isotope-assisted methods for quantifying glutamine metabolism in cancer cells
2014, Methods in EnzymologyOral citrulline does not affect whole body protein metabolism in healthy human volunteers: Results of a prospective, randomized, double-blind, cross-over study
2011, Clinical NutritionCitation Excerpt :Throughout isotope infusion citrulline or iso-nitrogenous placebo were administered as q. 20 min oral sips according to randomization. Breath 13CO2 enrichments were measured by gas chromatography-isotope ratio mass spectrometry (GC-IRMS; Isochrom III, VG, Ipswich, UK), and plasma 13C-α-keto-isocaproate enrichment by GCMS (Hewlett–Packard MSD 5971) as described.22 Leucine appearance rate into plasma (RaLEU; μmol kg−1 h−1), leucine oxidation (OxLEU; μmol kg−1 h−1) and non-oxidative leucine disposal (NOLD; μmol kg−1 h−1) were calculated using standard isotope dilution equations for steady state conditions as described.23
Determination of citrulline in human plasma, red blood cells and urine by electron impact (EI) ionization gas chromatography-mass spectrometry
2008, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
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This work was supported in part by National Institutes of Health Grants AM-25994, RR-00954, and RR-00036, the National Livestock and Meat Board, and le Ministere des Relations Exterieures, France.