Microanalysis of complex tissue lipids by high-performance thin-layer chromatography

doi:10.1016/0003-2697(85)90447-6

Analytical Biochemistry

Volume 150, Issue 1, October 1985, Pages 111-116

https://doi.org/10.1016/0003-2697(85)90447-6 Get rights and content

Abstract

We have developed a high-performance thin-layer chromatographic method, not involving preliminary column chromatography, for separating mixtures of nonpolar lipids, glycosphingolipids, and phospholipids in one dimension. Three or four consecutive developing solvent systems are used. More than 20 different lipid subclasses can be separated using this procedure. Reproducible results have been obtained by careful control of variables. In addition, a simple and rapid fractionation procedure, using Sep-Pak silica cartridges, has been developed to concentrate neutral glycosphingolipids from biological membranes, e.g., erythrocytes, so that patients with sphingolipidosis can be easily identified.

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^☆: This work was supported in part by Center Grants from the National Institute of Neurological and Communicative Disorders and Stroke (NS 14304) and the Muscular Dystrophy Association (MDA 12), by Mayo, Borchard, Upton and Gallagher funds, and by a grant from NINCDS (NS 18012).

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Microanalysis of complex tissue lipids by high-performance thin-layer chromatography☆

Abstract

J. Chromatogr

J. Lipid Res

Biochim. Biophys. Acta

J. Lipid Res

J. Neurol. Sci

Anal. Biochem

J. Lipid Res

J. Chromatogr