Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody
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Differential effects of intrastriatal 6-hydroxydopamine on cell number and morphology in midbrain dopaminergic subregions of the rat
2014, Brain ResearchCitation Excerpt :The antibody was rabbit polyclonal anti-tyrosine hydroxylase (Pel-Freez Biologicals, Rogers, AR; P40101-0, Lot# 06432; working dilution 1:1000), which was generated using SDS-denatured tyrosine hydroxylase purified from rat pheochromocytoma. The antibody was specific for a 60 kDa band on Western blots of rat striatal lysates (Haycock, 1989). The immunostaining pattern observed was also consistent with the reported distribution of tyrosine hydroxylase and its mRNA in rat brain (Chan and Sawchenko, 1998).
Noradrenaline increases pain facilitation from the brain during inflammatory pain
2013, NeuropharmacologyCitation Excerpt :To test the specificity of antisera, control experiments for β-gal were done using the same immunostaining protocol in brainstem sections of animals not injected with THZ. The specificity of the rat TH antiserum was previously demonstrated (Haycock, 1989). The specificity of the secondary antibodies was tested by omission of the primary antibodies.
Changes in serotoninergic and noradrenergic descending pain pathways during painful diabetic neuropathy: The preventive action of IGF1
2011, Neurobiology of DiseaseCitation Excerpt :The percentages of control animals were considered as the basal levels (equal to 1) and the results for STZ group are presented as fold-increase over the control percentages. The specificity of each primary antibody was previously demonstrated (Haycock, 1989; Pinto et al., 2007; Pinna et al., 2007; Riedel et al., 2008; Wu et al., 2009; Malleret et al., 2010). Negative control experiments included the omission of primary or secondary antibodies.
Reversal of neuropathic pain by HSV-1-mediated decrease of noradrenaline in a pain facilitatory area of the brain
2010, PainCitation Excerpt :For β-gal, the controls were done in brainstem sections of animals not injected with DPZ and using the same immunostaining protocol. The specificity of the rat TH antiserum was previously demonstrated [15]. The specificity of the secondary antibodies was tested by omission of the primary antibodies.
Quantification of MPTP-induced dopaminergic neurodegeneration in the mouse substantia nigra by laser capture microdissection
2007, Journal of Neuroscience MethodsDifferential expression of the B′βregulatory subunit of protein phosphatase 2A modulates tyrosine hydroxylase phosphorylation and catecholamine synthesis
2007, Journal of Biological ChemistryCitation Excerpt :Specificity was confirmed in heterologous expression experiments. Other Reagents—Rabbit polyclonal antibodies recognizing total TH and Ser19-phosphorylated TH were provided by John Haycock (Louisiana State University (30, 31)). The pGEX-2T plasmid expressing glutathione S-transferase (GST)-TH (regulatory domain residues 31-164) was a gift from Drs. Lily Moy and Li-Huei Tsai (Harvard University, Cambridge, MA (32)).