Elsevier

Brain Research

Volume 102, Issue 1, 30 January 1976, Pages 143-151
Brain Research

Transport of L-proline by rat brain slices

https://doi.org/10.1016/0006-8993(76)90580-1Get rights and content

Abstract

l-Proline is taken up into slices of rat cerebral cortex by a structurally specific ‘high affinity’ system which is absolutely dependent on sodium ions. The system mediating l-proline uptake in homogenates of cerebral cortex is associated with osmotically sensitive particles of the same equilibrium density as synaptosomes. Based on tissue-medium ratios, the uptake of l-proline is most efficient in slices of cerebral cortex and of hypothalamus, and least efficient in slices of cerebellum. l-Proline taken up into slices of cerebral cortex can be released from these slices by an increased potassium ion concentration in a calcium-dependent manner. These observations on l-proline transport into and out of brain slices are consistent with l-proline functioning as a synaptic transmitter in that they are analogous to observations on the transport of transmitter amino acids such as GABA.

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  • Cited by (0)

    *

    Present address: Centre de Neurochimie, Centre National de la Recherche Scientifique, Strasbourg, France.

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