WGA-HRP as a transneuronal marker in the visual pathways of monkey and rat
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Cited by (178)
Asymmetric cross-hemispheric connections link the rat anterior thalamic nuclei with the cortex and hippocampal formation
2017, NeuroscienceCitation Excerpt :Therefore, in two regions (the prelimbic cortex and the subiculum) that were not sufficiently covered by FB injections, we used WGA-HRP to enhance the retrograde component as well as to provide an anterograde signal. Survival times for WGA-HRP injections were kept relatively short (2 days) in order to limit the risk of trans-synaptic transport (Itaya and van Hoesen, 1982; Itaya et al., 1986; Itaya, 1987). Additional data came from injecting the retrograde tracers fast blue (FB) and diamidino yellow (DY) into the AV, AM and LD thalamic nuclei.
Visual System
2015, The Rat Nervous System: Fourth EditionNew technologies for imaging synaptic partners
2012, Current Opinion in NeurobiologyCitation Excerpt :While this allows tracing of multiple synaptic steps, it also obscures the number of synaptic steps that have been crossed, because different synapses are crossed at different rates [37,59]. When molecular tracers’ detection sensitivity is increased (e.g. by fusion with Cre [20] or by constitutive expression of genetically encoded forms [18]), labeling appears in an assortment of neurons whose synaptic relationships with the source neurons are unclear [15–17]. For viral tracers, although survival time is often used as a proxy for the number of synapses crossed [32,60], several reports demonstrate clear labeling of strong polysynaptic inputs before weak monosynaptic ones [37,56,61].
Genetic Dissection of Neural Circuits
2008, NeuronCitation Excerpt :Synapses made specifically by these neuronal populations could be detected based on GFP (Trachtenberg et al., 2002) or HRP (Watts et al., 2004) expression. Transneuronal tracers, including chemicals, proteins, and neurotropic viruses, have been used extensively for studies of neural circuits (Schwab et al., 1979; Fujisawa and Jacobson, 1980; Itaya and van Hoesen, 1982; Kelly and Strick, 2000; Enquist, 2002). Typically they are injected into a particular brain region and then allowed to spread either anterogradely or retrogradely through the circuit.
Seeing sounds: visual and auditory interactions in the brain
2006, Current Opinion in NeurobiologyVisual System
2004, The Rat Nervous System
This work was supported by a Fight for Sight Grant-in-Aid, Fight for Sight, Inc., New York City (S.K.I.), and NIH grant NS14944 (G.W.V.H.)
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We thank C. L. Barnes and D. Benjamin for technical assistance and helpful discussion.