Intracellular recording from salamander olfactory receptor cells

doi:10.1016/0006-8993(83)90488-2

Brain Research

Volume 268, Issue 2, 6 June 1983, Pages 225-237

https://doi.org/10.1016/0006-8993(83)90488-2 Get rights and content

Abstract

Intracellular recordings were obtained from salamander olfactory receptor cells. The occurrence of an intracellular spike in response to the antidromic stimulation of the olfactory fibers was considered as a physiological criterion of a neuronal impalement. The mean resting potential was−56 ± 9mV (mean± S.D.;n= 70). Fifty-two cells presented a spontaneous spike activi lower than 2 impulses/s. Appropriate olfactory stimulation generally evoked a slow and graded decrease (up to 28 mV) of the intracellular potential. The input resistance of the cell decreased markedly during the response. The slow potential change induced a repetitive firing. Increasing the intensity of the olfactory stimulation increased the instantaneous frequency of firing (up to 25 s⁻¹) are reduced the spike amplitude. The spike presented an inflexion in the rising phase indicating a two-stage depolarization. With the strongest intensities of stimulation the impulse activity was stopped during the repolarizing phase of the cell response when the membrane potential was still appreciably depolarized.

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Intracellular recording from salamander olfactory receptor cells

Abstract

J. theor. Biol.

Brain Research

Biophys. J.

An instrument for the production of microelectrodes used in electrophysiological studies

Rev. Sci. Instrum.

Intracellular recordings of the olfactory cell activity

Temporal patterns and selectivity in the unitary responses of olfactory receptors in the Tiger Salamander to odor stimulation

J. gen. Physiol.

Adaptation and cross-adaptation to odor stimulation of olfactory receptors in the Tiger Salamander

J. gen. Physiol.

Receptive fields of cones in the retinal of the turtle

J. Physiol. (Lond.)

Diffusion and the frog EOG

Intracellular recording from antidromically activated motoneurons

J. Physiol. (Lond.)

Significance of cilia and their motility in the functioning of olfactory receptors

Dokl. Akad. Nauk. U.S.S.R.

Regeneration of olfactory cilia and recovery of the electro-olfactogram after treatment of the frog olfactory mucosa with Triton X-100

Tsitologiya

The interpretation of spike potentials of motoneurons

J. Physiol. (Lond.)

Electro-olfactogramme local et discrimination olfactive chez la grenouille

J. Physiol. (Paris)

Analyse topographique de l'électro-olfactogramme chez la grenouille

J. Physiol. (Paris)

Olfactory nerve fibers

J. gen. Physiol.

Techniques for investigating single unit activity in the vertebrate olfactory epithelium

Chemical transmission in the nose of the frog

J. Physiol. (Lond.)

Analysis of unitary spikes recorded ectracellularly from frog olfactory cells and axons

J. Physiol. (Lond.)

Electrogenic sources of slow voltage transients recorded from frog olfactory epithelium

J. Neurophysiol.

Responses of olfactory receptor cells to step pulses of odour at different concentrations in the Salamander

J. Physiol. (Lond.)

Olfactory epithelium ofNecturus maculosus andAmbystoma tigrinum

J. Neurocytol.