Double-labeling of neuropeptide Y-immunoreactive neurons which project from the geniculate to the suprachiasmatic nuclei

Abstract

A projection from the ventral geniculate area to the suprachiasmatic nuclei (SCN) has been demonstrated in rats and hamsters. Large lesions in this area of the geniculate cause a dramatic decrease in neuropeptide Y-immunoreactivity in the SCN. Since numerous neuropeptide Y-immunoreactive neurons are found in the lateral geniculate area, we and others proposed that these immunoreactive neurons project to the SCN. In the present study, neurons in the lateral geniculate area of golden hamster brains were examined for both neuropeptide Y-immunoreactivity and a retrograde tracer transported from the SCN. Two days after a pressure injection of wheat germ agglutinin-horseradish peroxidase (WGA-HRP) into the SCN of hamsters, labeled neurons were found in the intergeniculate leaflet and in the external lamina of the anterior ventral lateral geniculate nucleus (VLGN). These neurons were compared with similarly located neurons which showed immunoreactivity for neuropeptide Y. Morphometric comparisons of neuropeptide Y- and WGA-HRP-labeled neurons indicated that they were comparable in terms of soma size, number of dendrites, orientation and localization. In additional hamsters, neurons double-labeled with a retrograde tracer and neuropeptide Y-immunoreactivity were localized in the intergeniculate leaflet and in the external lamina of the anterior VLGN. These results demonstrate that many neuropeptide Y-immunoreactive neurons located in both the intergeniculate leaflet and in the external lamina of the anterior VLGN project to the SCN in hamsters.