Dopaminergic neurons in the nucleus raphe dorsalis innervate the prefrontal cortex in the rat: a combined retrograde tracing and immunohistochemical study using anti-dopamine serum

doi:10.1016/0006-8993(89)91091-3

Brain Research

Volume 496, Issues 1–2, 4 September 1989, Pages 373-376

https://doi.org/10.1016/0006-8993(89)91091-3 Get rights and content

Abstract

The existence of a dopaminergic projection from the nucleus raphe dorsalis (RD) to the prefrontal cortex of the rat was demonstrated by combining immunohistochemistry with retrograde tracing. Injection of horseradish peroxidase or Fluoro-gold into the medial prefrontal cortex resulted in the appearance of retrogradely labelled neurons in the RD and in other brain regions. Some of these retrogradely labelled RD neurons were shown to be immunoreactive for dopamine. These data support the view that these dopaminergic RD neurons represent an extension of the A10 cell group.

References (18)

GeffardM. et al.
First demonstration of highly specific and sensitive antibodies against dopamine
Brain Research
(1984)
LidovH.G.W. et al.
The serotonin innervation of the cerebral cortex in the rat—an immunohistochemical analysis
Neuroscience
(1980)
LindvallO. et al.
Organization of catecholamine neurons projecting to the frontal cortex in the rat
Brain Research
(1978)
OchiJ. et al.
Occurrence of dopamine-containing neurons in the midbrain raphe nuclei of the rat
Neurosci. Lett.
(1978)
SchmuedL.C. et al.
Fluorogold: a new fluorescent retrograde axonal tracer with numerous unique properties
Brain Research
(1986)
TrulsonM.E. et al.
Identification of dopamine-containing cell bodies in the dorsal and median raphe nuclei of the rat using tyrosine hydroxylase immunohistochemistry
Brain Res. Bull.
(1985)
YoshidaM. et al.
Existence of new dopaminergic terminal plexus in the rat spinal cord: assessment by immunohistochemistry using anti-dopamine serum
Neurosci. Lett.
(1988)
DescarriesL. et al.
Dopaminergic projection from nucleus raphe dorsalis to neostriatum in the rat
J. Comp. Neurol.
(1986)
HökfeltT. et al.
Distribution maps of tyrosine-hydroxylase-immunoreactive neurons in the rat brain

There are more references available in the full text version of this article.

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: Supported in part by the Medical Research Council of Canada (PG-23).

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Dopaminergic neurons in the nucleus raphe dorsalis innervate the prefrontal cortex in the rat: a combined retrograde tracing and immunohistochemical study using anti-dopamine serum

Abstract

Brain Research

Neuroscience

Brain Research

Neurosci. Lett.

Brain Research

Brain Res. Bull.

Neurosci. Lett.

Dopaminergic projection from nucleus raphe dorsalis to neostriatum in the rat

J. Comp. Neurol.

Distribution maps of tyrosine-hydroxylase-immunoreactive neurons in the rat brain