Glutamate-induced overexpression of NMDA receptor messenger RNAs and protein triggered by activation of AMPA/kainate receptors in rat hippocampus following forebrain ischemia

doi:10.1016/0006-8993(94)90864-8

Brain Research

Volume 659, Issues 1–2, 3 October 1994, Pages 67-74

https://doi.org/10.1016/0006-8993(94)90864-8 Get rights and content

Abstract

Severe forebrain ischemia induces a large increase in expression of NMDA receptor subunits in rat brain. One week after ischemia, levels of NMDA-R1 mRNAs in the CA1 pyramidal cells of hippocampus are 7 times higher than those observed in control rats. At 7 days postischemia, an enhanced immunostaining of the NMDA-R1 subunit was observed in all hippocampal structures indicating that changes in mRNA levels are accompanied by changes in receptor protein level. Riluzole, a potent inhibitor of glutamate release and CNQX, a selective AMPA/kainate antagonist, drastically reduced the ischemia-induced expression of mRNAs for three NMDA receptor subunits while d-AP5, a selective NMDA antagonist, had essentially no effect. Therefore ischemia-induced expression of NMDA receptor subunits is associated with glutamate release and proceeds via an AMPA/kainate pathway. These results together with those of other groups concerning ischemia effects on AMPA and GABA _A receptor levels, suggest an important role of the induced expression of NMDA receptor subunits in the deleterious effects of ischemia.

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Glutamate-induced overexpression of NMDA receptor messenger RNAs and protein triggered by activation of AMPA/kainate receptors in rat hippocampus following forebrain ischemia

Abstract

Neurosci. Lett.

J. Biol. Chem.

Neurosci. Lett.

Neuron

Neurosci. Lett.

Brain Res.

Neuropharmacology

Neuron

Neuroscience

Neurosci. Lett.

Eur. J. Pharmacol.

J. Biol. Chem.

Eur. J. Pharmacol.

Brain Res.

Eur. J. Pharmacol.

Brain Res.

Curr. Op. Neurobiol.

Oncogene jun encodes a sequence-specific trans-activator similar to AP-1

Nature

Elevation of the extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis

J. Neurochem.

Molecular cloning and functional expression of glutamate receptor subunit genes

Science

Isolation and characterization of the c-fos (rat) cDNA and analysis of post-translational modification in vitro

Oncogene