Elsevier

Brain Research

Volume 655, Issues 1–2, 29 August 1994, Pages 25-32
Brain Research

Research report
Chronic nicotine treatment counteracts dopamine D2 receptor upregulation induced by a partial meso-diencephalic hemitransection in the rat

https://doi.org/10.1016/0006-8993(94)91593-8Get rights and content

Abstract

To further elucidate the previously demonstrated protective actions of nicotine on lesioned nigrostriatal dopamine (DA) systems (Janson and Møller, Neuroscience, 57 (1993) 931–941), the present receptor binding experiments were carried out. Rats were partially hemitransected at the meso-diencephalic junction and the effects of chronic continuous (-)nicotine treatment (osmotic pumps s.c., 0.125 mg/kg/h, 14 days) on [3H]N-propylnorapomorphine ([3H]NPA) and [3H]methylcarbamylcholine ([3H]MCC) binding were investigated in striatal coronal sections to study the agonist binding sites of DA D2 receptors and nicotinic cholinoceptors, respectively. In saline-treated but not in nicotine-treated rats, the lesion led to an increased Bmax value of [3H]NPA binding. The Bmax value of [3H]MCC binding was increased by nicotine treatment and decreased by the partial hemitransection. These results indicate that chronic nicotine treatment counteracts the lesion-induced upregulation of the high-affinity agonist binding site of the DA D2 receptor, which may be explained by an increased presence of DA via a protective effect of nicotine on neostriatal DA terminals. This action of nicotine may be of interest in the treatment of neurodegenerative diseases such as Parkinson's disease.

References (40)

  • J.N. Joyce et al.

    Striatal topography of D-2 receptors correlates with indexes of cholinergic neuron localization

    Neurosci. Lett.

    (1985)
  • C. Köhler et al.

    Specific in vitro and in vivo binding of 3H-raclopride: a potent substituted benzamine drug with high affinity for dopamine D2 receptors in the rat brain

    Biochem. Pharmacol.

    (1985)
  • L.L. Miner et al.

    Effect of nicotine pre-treatment on nicotine-induced seizures

    Pharmacol. Biochem. Behav.

    (1988)
  • Ch. Owman et al.

    Chronic nicotine treatment eliminates asymmetry in striatal glucose utilization following unilateral transection of the mesostriatal dopamine pathway in rats

    Neurosci Lett.

    (1989)
  • E. Senba et al.

    RNA levels of neuronal nicotinic acetylcholine receptor subunits are differentialy regulated in axotomized facial motoneurons: an in situ hybridization study

    Mol. Brain Res.

    (1990)
  • L.F. Agnati et al.

    Gangliosides increase the survival of lesioned nigral dopamine neurons and favour the recovery of dopaminergic synaptic function in striatum of rats by collateral sprouting

    Acta Physiol. Scand.

    (1983)
  • L.F. Agnati et al.

    Computer assisted morphometry and microdensitometry of transmitter identified neurons with special reference to the mesostriatal dopamine pathway. Methodological aspects

    Acta Physiol. Scand.

    (1984)
  • J.A. Baron

    Cigarette smoking and Parkinson's disease

    Neurology

    (1986)
  • F. Benfenati et al.

    Quantitative autoradiography of central neurotransmitter receptors: methodological and statistical aspects with special reference to computer-assisted image analysis

    Acta Physiol. Scand.

    (1986)
  • J.P. Changeux et al.

    The acetylcholine receptor: functional architecture and regulation

    Adv. Second Messenger Phosphoprotein Res.

    (1990)
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