Full paperLocalization of vimentin, the nonspecific intermediate filament protein, in embryonal glia and in early differentiating neurons: In vivo and in vitro immunofluorescence study of the rat embryo with vimentin and neurofilament antisera☆
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2019, Journal of Comparative PathologyMorphological development of the human cochlear nucleus
2019, Hearing ResearchCitation Excerpt :Thereafter, nearer term, the number of the CV-identifiable and NeuN-immunostained neurons were almost identical and difference between the two methods was not significant (p > 0.05). We also used immunohistochemical expression of GFAP to identify the glial cell that is key during development (Bignami et al., 1982; Dahl et al., 1981). We observed a heterogenous pattern of GFAP-immunostaining in the CNC, having more intense staining at the pial surface.
Immunomorphometric variations of sustentacular cells of the male viscacha adrenal medulla during the annual reproductive cycle. Effects of androgens and melatonin
2018, Acta HistochemicaCitation Excerpt :In the peripheral nervous system, the expression of GFAP has been observed in non-myelinating Schwann cells and in sustentacular cells of rat adrenal medulla (Suzuki and Kachi, 1995). Vimentin, initially described as a characteristic component of mesenchymal cells, has been observed in different cell types in a range of maturation states, particularly in immature glial cells where this protein constitutes a major component of the cytoskeleton (Bignami et al., 1982; Schnitzer et al., 1981). Our experimental model, the viscacha (Lagostomus maximus maximus), is the largest member of the Chinchillidae family.
Using Xenopus Embryos to Study Transcriptional and Posttranscriptional Gene Regulatory Mechanisms of Intermediate Filaments
2016, Methods in EnzymologyCitation Excerpt :This characteristic has led to the extensive use of intermediate filament mRNA and protein expression both as identifiers of the origins of metastasized tumors (Bannasch, Zerban, Schmid, & Franke, 1980) and as markers for the state of differentiation of specific cell types (Cochard & Paulin, 1984; Godsave, Anderton, & Wylie, 1986; Tapscott, Bennett, Toyama, Kleinbart, & Holtzer, 1981). In developing mammalian neurons, for example, undifferentiated neuroepithelial cells initially express nestin (Lendahl, Zimmerman, & McKay, 1990), followed by vimentin (Bignami, Raju, & Dahl, 1982; Cochard & Paulin, 1984). As neuroblasts of the central nervous system (CNS) differentiate into neurons, expression of these intermediate filament genes is followed first by expression of α-internexin (Kaplan, Chin, Fliegner, & Liem, 1990), and of peripherin in phylogenetically old CNS neurons (Escurat, Djabali, Gumpel, Gros, & Portier, 1990; Parysek & Goldman, 1988; Troy, Brown, Greene, & Shelanski, 1990), together with low levels of the light (nefl) and medium (nefm) neurofilament triplets (Carden, Trojanowski, Schlaepfer, & Lee, 1987; Nixon & Shea, 1992).
Identification of target antigens of naturally occurring autoantibodies in cerebrospinal fluid
2015, Journal of ProteomicsCitation Excerpt :Vimentin is a type III intermediate filament protein that is expressed in mesenchymal cells. In the healthy adult brain, vimentin is not present in neurons and restricted to vascular endothelial cells and specific subpopulations of glial cells [26]. However, vimentin is expressed in neurons undergoing neuronal differentiation and neurite extension in the human fetal brain [27].
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This work was supported by National Science Foundation Grant BNS-791296, USPHS Grant NS 13034, and by the Veterans Administration.