Neuronal acquisition of tetanus toxin binding sites: Relationship with the last mitotic cycle

doi:10.1016/0012-1606(83)90229-4

Developmental Biology

Volume 100, Issue 2, December 1983, Pages 350-357

https://doi.org/10.1016/0012-1606(83)90229-4 Get rights and content

Abstract

In an earlier study on the developing nervous system, the existence of a temporal correlation between the appearance of tetanus toxin-binding cells and neurogenesis was reported (A. Koulakoff, B. Bizzini, and Y. Berwald-Netter (1982). Dev. Brain Res. 5, 139–147). Using a combined approach of immunocytochemistry and [³H]thymidine autoradiography it is shown that, in the fetal mouse central nervous system, dividing cells do not express membrane binding sites for tetanus toxin. A time-course quantitative autoradiography revealed that the toxin-binding sites become apparent within 7 ± 1 hr, following the last S phase, on cells undergoing the conversion from dividing to postmitotic state. The acquisition of surface binding sites for tetanus toxin may thus be an early property of nascent central neurons, marking the transition from cycling precursor neuroblasts to postmitotic neuronal cells. Parallel studies on in vivo-developing dorsal root ganglia disclosed that at least some peripheral nervous system cells are endowed with tetanus toxin-binding capacity while still capable of DNA synthesis and undergo one or more divisions.

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^☆: This study was supported by the Centre National de la Recherche Scientifique and the Ministère de la Recherche et de la Technologie (A.T.P.: Dynamique du Neurone).

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Full paperNeuronal acquisition of tetanus toxin binding sites: Relationship with the last mitotic cycle☆

Abstract

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Full paper
Neuronal acquisition of tetanus toxin binding sites: Relationship with the last mitotic cycle☆