Distribution and ontogeny of SP, CGRP, SOM, and VIP in chick sensory and sympathetic ganglia

doi:10.1016/0012-1606(86)90137-5

Developmental Biology

Volume 116, Issue 2, August 1986, Pages 337-346

https://doi.org/10.1016/0012-1606(86)90137-5 Get rights and content

Abstract

The distribution and ontogeny of four neuropeptides in developing chick lumbosacral sensory and sympathetic ganglia were studied using immunohistochemical techniques. Antibodies to two of these peptides, substance P (SP) and calcitonin gene-related peptide (CGRP), stained small neurons in the medial part of the dorsal root ganglia from embryonic Day 5 and Day 10, respectively, whereas neurons in the lateral part of the ganglia were negative; this distribution persisted throughout development. Both sets of neurons apparently send fibers to the dorsal horn of the spinal cord: SP to laminae I and II, and CGRP to lamina I, suggesting that the SP- and CGRP-positive sensory neurons are nociceptive or thermoreceptive. This correlation between the presence of SP or CGRP in a neuron and a particular functional modality thus provides evidence for a functional distinction between the mediodorsal and ventrolateral zones that are apparent during the development of chick dorsal root ganglia. Moreover, this study suggests that the type of neuron that develops within the dorsal root ganglion correlates with its position within the ganglion. In contrast to SP and CGRP, somatostatin (SOM) and vasoactive intestinal polypeptide (VIP) immunoreactivities were not seen in the lumbosacral sensory ganglia at any stage during development. However, both were present in sympathetic ganglia: SOM from embryonic Day 4.5 and VIP from embryonic Day 10. VIP immunoreactivity persisted throughout development in a large number of sympathetic neurons, but the number of cells with SOM immunoreactivity decreased from embryonic Day 10 onward. SOM therefore appears to be present only transiently in most chick lumbosacral sympathetic cells.

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¹: H.V.N. was supported by a Nuffield Small Grant and a Medical Research Council Research Studentship.

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Full paperDistribution and ontogeny of SP, CGRP, SOM, and VIP in chick sensory and sympathetic ganglia

Abstract

Cell Differ

Dev. Biol

Neuroscience

Dev. Biol

Dev. Biol

The origin, distribution and synaptic relationships of substance P axons in rat spinal cord

J. Comp. Neurol

Organization in the Spinal Cord

Proliferative and degenerative events in the early development of chick dorsal root ganglia

J. Comp. Neurol

Substance P-containing pathways to avian sympathetic preganglionic neurons: Evidence for major spinal-spinal circuitry

J. Neurosci

In vivo and in vitro development of somatostatin-like-immunoreactivity in the peripheral nervous system of quail embryos

J. Neurosci

Calcitonin generelated peptide immunoreactivity in the spinal cord of man and eight other species

J. Neurosci

Proliferation, differentiation and degeneration in the spinal ganglia of the chick embryo under normal and experimental conditions

J. Exp. Zool

A series of normal stages in the development of the chick embryo

J. Morphol

Neuronal death in the spinal ganglia of the chick embryo and its reduction by nerve growth factor

J. Neurosci

Immunohistochemical evidence for separate populations of somatostatin-containing and substance P-containing primary afferent neurons in the rat

Neuroscience

The development of sensory projection patterns in embryonic chick hind limb

J. Physiol. (London)

Full paper
Distribution and ontogeny of SP, CGRP, SOM, and VIP in chick sensory and sympathetic ganglia