Platelets as a source of fibroblast growth-promoting activity☆,☆☆
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2022, Analytical BiochemistryCitation Excerpt :Fetal serum contain factors that promote changes in gene expression [1] and permit the indefinite culture of cell lines [2–4] such as RAW 264.7 murine macrophages [5]. Insulin [6], nerve growth factor [7], epidermal growth factor [8], insulin-like growth factor [9,10], fibroblast growth factor (FGF) [2,11] and platelet-derived growth factor [3,12,13] have been shown to regulate cellular proliferation. However, the effect of known growth factors was inferior to that of fetal serum even after supplementation with albumin, transferrin, amino acids or dilute serum [14], therefore, all of the factors that permit indefinite cell growth have yet to be determined [15–18].
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This work was supported by a grant from the American Cancer Society (VC no. 78) (Edith C. Hanna Memorial Grant For Cancer Research) and by USPHS grant no. CA-15858 from NCI.
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An abstract of this work was presented at the meeting of the American Society for Microbiology, Chicago, Ill., May 1974.