Platelets as a source of fibroblast growth-promoting activity

doi:10.1016/0014-4827(74)90484-4

Experimental Cell Research

Volume 87, Issue 2, August 1974, Pages 297-301

https://doi.org/10.1016/0014-4827(74)90484-4 Get rights and content

Abstract

Evidence is presented that platelets are an enriched source of growth-promoting activity for 3T3 cells, an established line of mouse fibroblasts. Rat or human serum stimulates 3T3 cell growth more than autologous plasma; this increased activity is at least partly due to the release of a growth-promoting activity from platelets disrupted during the clotting process. Platelet extract stimulation of 3T3 cell growth is approx. 5 times greater than an equal amount of autologous serum. This platelet growth activity is (1) pH-stable; (2) pepsin-labile; (3) heat-stable at 56 °C; (4) non-dialysable, and (5) similar in size to the serum 3T3 growth-promoting molecule. 3T3 cells transformed by Simian virus 40 show an equivalent stimulation by the platelet solution and serum.

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^☆: This work was supported by a grant from the American Cancer Society (VC no. 78) (Edith C. Hanna Memorial Grant For Cancer Research) and by USPHS grant no. CA-15858 from NCI.

^☆☆: An abstract of this work was presented at the meeting of the American Society for Microbiology, Chicago, Ill., May 1974.

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Platelets as a source of fibroblast growth-promoting activity☆,☆☆

Abstract

Exptl cell res

Growth regulating substances for animal cells in culture