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Cited by (86)
Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells
2016, CellCitation Excerpt :Sequences for primers for the production of mouse antisense RNA probes were obtained from Allen Institute for Brain Science, and are given in the Key Resources Table. In situ hybridization was performed using Digoxigenin-labeled probes and detection with alkaline phosphatase-conjugated antibody, as described previously (Conlon and Herrmann, 1993). Briefly, 4% paraformaldehyde preserved and cryo-embedded embryos were sectioned coronally at 14 μm.
Oxygen tension regulates chondrocyte differentiation and function during endochondral ossification
2006, Journal of Biological ChemistryCitation Excerpt :Digitized pictures taken for each radius were analyzed to calculate the ratio of the area stained with safranin-O per whole radius. In situ hybridization for Col10a1 was done as described previously (53, 57). The proportional length of the hypertrophic positive signal of Col10a1 with respect to the whole hypertrophic chondrocyte zone along the midline was calculated.
Preparation and application of digoxin-labeled Nkx2. 1 gene RNA probe
2019, Chinese Journal of Biologicals