Cell
Volume 23, Issue 1, January 1981, Pages 175-182
Journal home page for Cell

Article
SV40-transformed simian cells support the replication of early SV40 mutants

https://doi.org/10.1016/0092-8674(81)90282-8Get rights and content

Abstract

CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1, -3, -7) were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40°C; and support the replication of pure populations of SV40 mutants with deletions in the early region. One of the lines (COS-1) contains a single integrated copy of the complete early region of SV40 DNA. These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.

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