Cell
Volume 35, Issue 2, Part 1, December 1983, Pages 551-559
Journal home page for Cell

Article
Modulations of neurofilament axonal transport during the development of rabbit retinal ganglion cells

https://doi.org/10.1016/0092-8674(83)90189-7Get rights and content

Abstract

We have compared the polypeptides undergoing axonal transport in the retinal ganglion cells of neonatal and adult rabbits, and have observed the following: (1) Representative polypeptides of five different adult transport groups are axonally transported from the time of birth. (2) Polypeptides of group IV (a group that includes actin and myosin) are transported two-fold more rapidly in neonates than in adults. (3) Two polypeptides, M (145K) and L (73K) that are components of neurofilaments and move with the fifth, slowest group of transported proteins, are transported approximately eight-fold more rapidly in neonatal rabbits than in adults. (4) H, a third group V polypeptide, that serves to crosslink neurofilaments, was not detected in the rabbit optic nerve until 12 days after birth. We consider the possibility that the late induction of the crosslinker precipitates a cytoskeletal “phase transition” that may be responsible for the developmental alterations in apparent transport velocities, and may have additional consequences for neuronal development.

References (39)

  • R.B. Wuerker et al.

    Neurofilaments and microtubules in anterior horn cells of the rat

    Tissue and Cell

    (1969)
  • C. Baitinger et al.

    Characteristics of axonally transported proteins

  • M.H. Ellisman et al.

    Microtrabecular structure of the axoplasmic matrix: visualization of cross-linking structures and their distribution

    J. Cell Biol.

    (1980)
  • N. Hirokawa

    Cross-linker system between neurofilaments, microtubules, and membrane organelles in frog axons revealed by the quick-freeze, deep-etching method

    J. Cell Biol.

    (1982)
  • P.N. Hoffman et al.

    The slow component of axonal transport

  • V.K. Laemmli

    Cleavage of structural proteins during the assembly of the head of bacteriophage T4

    Nature (Lond.)

    (1970)
  • J.-O. Karlsson et al.

    Synthesis, migration and turnover of protein in retinal ganglion cells

    J. Neurochem.

    (1971)
  • R.J. Lasek

    The dynamic ordering of neuronal cytoskeletons

    Neurosci. Res. Prog. Bull.

    (1981)
  • J. Levine et al.

    Fodrin: axonally transported polypeptides associated with the internal periphery of many cells

    J. Cell Biol.

    (1981)
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