Research paperRat hippocampal slices ‘in vitro’ display spontaneous epileptiform activity following long-term organotypic culture
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Imaging pathological activities of human brain tissue in organotypic culture
2018, Journal of Neuroscience MethodsCitation Excerpt :Ca-imaging suggested that single cells directly excited a minority of potential post-synaptic partners (Fig. 6). Furthermore, even if neuronal excitability was increased at 40–50 DIV (Fig. 1C), epileptiform activities did not change greatly with time in culture (McBain et al., 1989; Lillis et al., 2015) as might expected if there were a strong genesis of new recurrent synapses. These techniques will facilitate new approaches to questions on human neurological disorders.
Organotypic Hippocampal Slice Cultures as a Model of Posttraumatic Epileptogenesis
2017, Models of Seizures and Epilepsy: Second EditionAn organotypic brain slice preparation from adult patients with temporal lobe epilepsy
2014, Journal of Neuroscience MethodsContribution of Aberrant GluK2-Containing Kainate Receptors to Chronic Seizures in Temporal Lobe Epilepsy
2014, Cell ReportsCitation Excerpt :Reliable stereotyped spontaneous interictal-like activity (Sabolek et al., 2012) was recorded in cultured slices using extracellular field recordings in 5 mM K+-containing ACSF and 5 μM gabazine (Figures 1B and 1C). The interical-like activity consisted of two distinct phases, the paroxysmal discharge (PD) followed by a late phase containing recurrent bursts (RBs) (McBain et al., 1989; Gutiérrez and Heinemann, 1999; Thomas et al., 2005; Dyhrfjeld-Johnsen et al., 2010) (Figure 1C). The number of interictal-like events was markedly reduced in cultured slices from GluK2−/− mice in comparison with WT (events/min: 3.2 ± 0.4 in WT and 1.7 ± 0.3 in GluK2−/−) (Figure 1B).
Network Control Mechanisms-Synaptogenesis and Epilepsy Development
2014, Neuronal Networks in Brain Function, CNS Disorders, and Therapeutics
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