Subcellular localization of α-tubulin and opsin mRNA in the goldfish retina using digoxigenin-labeled cRNA probes detected by alkaline phosphatase and HRP histochemistry

doi:10.1016/0165-0270(93)90002-9

Journal of Neuroscience Methods

Volume 50, Issue 2, November 1993, Pages 145-152

https://doi.org/10.1016/0165-0270(93)90002-9 Get rights and content

Abstract

This paper describes a method for non-radioactive in situ hybridization providing subcellular localization of mRNA in 3 μm cryosections. We used two alternative colorimetric reactions to detect digoxigenin-labeled cRNA probes: alkaline phosphatase and HRP (horseradish peroxidase). With some probes the signal with the alkaline phosphatase reaction was intense, and diffusion of the reaction product was noticeable. Using HRP-conjugated antibodies improved the resolution but decreased the sensitivity of the signal. Photoamplification of the HRP reaction product increased the contrast and improved the sensitivity of the technique.

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Research paperSubcellular localization of α-tubulin and opsin mRNA in the goldfish retina using digoxigenin-labeled cRNA probes detected by alkaline phosphatase and HRP histochemistry

Abstract

Pharmacol. Res.

Neuron

J. Neurosci. Methods

J. Neurosci. Methods

Neuron

Tetrazolium salts and formazans

Prog. Histochem. Cytochem.

Improved method for obtaining 3-μm cryosections for immunocytochemistry

J. Histochem. Cytochem.

Double labeling with non-isotopic in situ hybridization and BrdU immunohistochemistry: calmodulin (CaM) mRNA expression in post-mitotic neurons of the olfactory system

J. Histochem. Cytochem.

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

Nucleic Acids Res.

Resolution and limitations of the immunoperoxidase procedure in the localization of extracellular matrix antigens

J. Histochem. Cytochem.

In situ detection of low abundance transcripts of the myogenic factor qmf1 and myosin heavy chain protein in quail embryos

BioTechniques

Localization of specific mRNAs in Xenopus embryos by whole-mount in situ hybridization

Development

Research paper
Subcellular localization of α-tubulin and opsin mRNA in the goldfish retina using digoxigenin-labeled cRNA probes detected by alkaline phosphatase and HRP histochemistry