Characterization of glial cultures from rapid autopsies of Alzheimer's and control patients

doi:10.1016/0197-4580(96)00006-1

Neurobiology of Aging

Volume 17, Issue 3, May–June 1996, Pages 421-429

https://doi.org/10.1016/0197-4580(96)00006-1 Get rights and content

Abstract

We have developed isolated and mixed cultures of microglia, astrocytes, and oligodendrocytes from rapid (mean of 2 h 55 min) autopsies of nondemented elderly patients and patients with Alzheimer's disease, Parkinson's disease, and multiple sclerosis. Cultures were derived from both the corpus callosum (CC) and superior frontal gyrus (SFG). Cultured microglia phagocytosed latex beads, were reactive for DiI-acetylated low density lipoprotein, were immunoreactive for CD68 and major histocompatibility complex II markers, and were not immunoreactive for fibroblast, astrocyte, or oligodendrocyte markers. Cultured astrocytes included fibrous and protoplasmic types, were immunoreactive for GFAP, and were not immunoreactive for fibroblast, microglia, or oligodendrocyte markers. Cultured oligodendrocytes were poorly adherent, were slow to develop, were immunoreactive for galactocerebroside, and were not immunoreactive for fibroblast, microglia, or astrocyte markers. Because they are readily manipulated under controlled experimental conditions, and because they permit immediate access to individual cells and sets of cells from patients who have actually suffered the disease, these cultures may provide an important new tool for unravelling the etiology and pathogenesis of human CNS disorders.

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ArticleCharacterization of glial cultures from rapid autopsies of Alzheimer's and control patients

Abstract

Brain Res.

Dev. Brain Res.

Brain Res.

J. Neuroimmunol.

J. Neuroimmunol.

J. Neuroimmunol.

J. Neuroimmunol.

Neurosci. Lett.

J. Neuroimmunol.

J. Neurol. Sci.

Neuron

Differentiation

Int. J. Dev. Neurosci.

Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2

Lab. Invest.

Preoligodendrocytes from adult human CNS

J. Neurosci.

Morphological differentiation and proteoglycan synthesis regulate Alzheimer amyloid precursor protein processing in PC-12 and human astrocyte cultures

J. Neurosci. Res.

Effects of interleukin-1 and interleukin-6 on metallothionein and amyloid precursor protein expression in human neuroblastoma cells

J. Neuroimmunol.

CNS glial scar tissue: A source of molecules which inhibit central neurite outgrowth

Astrocytes and microglia in human brain share an epitope recognized by a B-lymphocyte specific monoclonal antibody (LN-1)

Am. J. Pathol.

Neuropathological changes in scrapie and Alzheimer's disease are associated with increased expression of apolipoprotein E and cathepsin D in astrocytes

J. Virol.

Glial and neuronal changes in experimental hepatic encephalopathy

Acta Neurol. Scand.

Astrocytic response to injury

Article
Characterization of glial cultures from rapid autopsies of Alzheimer's and control patients