Horseradish peroxidase study in rat of the neural connections of the organum vasculosum of the lamina terminalis

doi:10.1016/0304-3940(81)90391-8

Neuroscience Letters

Volume 25, Issue 3, 25 September 1981, Pages 201-204

https://doi.org/10.1016/0304-3940(81)90391-8 Get rights and content

Abstract

Using highly localized injections by a transbuccal approach a study of the afferent and efferent connections of the organum vasculosum laminae terminalis (OVLT) with horseradish peroxidase (HRP) methods was carried out. The results showed that the OVLT has direct connections to several hypothalamic nuclei (anterior, preoptic, lateral preoptic, ventromedial nucleus) and to extrahypothalamic (central gray, locus coeruleus, subfornical organ) regions. There was not a direct projection from the supraoptic nucleus. Some of these connections may be involved in the functional actions of the OVLT observed after central injections of angiotensin II.

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There are more references available in the full text version of this article.

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Citation Excerpt :
Polysynaptic pathway to the suprachiasmatic nuclei was also showed by retrograde viral tracing [82]. OVLT receives other afferences than from the HT nuclei as from locus ceruleus and the grey perimesencephalic substance [83]. An afferent polysynaptic pathway from the kidney passing through HT was also been showed [82].
Circumventricular organs (CVOs) are a diverse group of specialised structures characterized by peculiar vascular and position around the third and fourth ventricles of the brain. In humans, these organs are present during the fetal period and some become vestigial after birth. Some, such as the pineal gland (PG), subcommissural organ (SCO) and organum vasculosum of the lamina terminalis (OVLT), which are located around the third ventricle, might be the site of origin of periventricular tumours. In contrast to humans, CVOs are present in the adult rat and can be dissected by laser capture microdissection (LCM).
In this study, we used LCM and microarrays to analyse the transcriptomes of three CVOs, the SCO, the subfornical organ (SFO) and the PG and the third ventricle ependyma of the adult rat, in order to better characterise these organs at the molecular level. Furthermore, an immunohistochemical study of Claudin-3 (CLDN3), a membrane protein involved in forming cellular tight junctions, was performed at the level of the SCO.
This study highlighted some potentially new or already described specific markers of these structures as Erbb2 and Col11a1 in ependyma, Epcam and CLDN3 in the SCO, Ren1 and Slc22a3 in the SFO and Tph, Anat and Asmt in the PG. Moreover, we found that CLDN3 expression was restricted to the apical pole of ependymocytes in the SCO.
Les organes circumventriculaires (OCV) sont des régions spécialisées de l’épendyme situées autour du troisième et du quatrième ventricule avec une vascularisation particulière. Mieux connus chez l’animal, certains OCV sont atrophiés chez l’homme où ils sont présents le plus souvent sous forme vestigiale. Certains OCV comme la glande pinéale (GP), l’organe sous-commissural (OSC) et l’organe vasculaire de la lame terminale (OVLT), peuvent être à l’origine des tumeurs périventriculaires. Contrairement à l’homme, les OCV chez le rat sont présents à l’âge adulte et peuvent être étudiés par des techniques de microdissection laser (MDL).
Dans cette étude, nous avons utilisé les techniques de MDL et de microarray pour étudier le transcriptome de trois OCV : OSC, l’organe subfornical (OSF) et GP ainsi que celui de l’épendyme du troisième ventricule chez le rat adulte. Nous avons également réalisé une étude immunohistochimique de la Claudine-3 (CLDN3), une protéine membranaire impliquée dans la formation des jonctions serrées au niveau de l’OSC.
Nous avons mis en évidence au niveau des OCV analysés des marqueurs moléculaires nouveaux ou déjà montrés dans ces structures comme Erbb2 et Col11a1 dans l’épendyme, Epcam et CLDN3 dans l’OSC, Ren1 et Slc22a3 dans l’OSF et Tph, Anat et Asmt dans la GP. De plus, l’étude immunohistochimique a montré une expression de CLND3 uniquement au niveau du pôle apical des épendymocytes de l’OSC.

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Horseradish peroxidase study in rat of the neural connections of the organum vasculosum of the lamina terminalis

Abstract

The role of the anteroventral third ventricle (AV3V) region in experimental hypertension

Circulat. Res.

Observations of labeling of neuronal cell bodies, axons and terminal after injection of horseradish peroxidase into rat brains

J. comp. Neurol.

Zur Morphologie des circumventrikularen Organe des Zwischenhirnes der Saugetiere

Verh. Dtsch. Zool. Ges.

Immunocytology of LHRH neurons