Elsevier

Neuroscience Letters

Volume 159, Issues 1–2, 3 September 1993, Pages 183-186
Neuroscience Letters

Cloning and expression of a glutamate transporter from mouse brain

https://doi.org/10.1016/0304-3940(93)90829-AGet rights and content

Abstract

A complementary DNA clone encoding a transporter for glutamate has been isolated from mouse brain, and its functional properties have been examined in Xenopus oocytes. The transporter displays high affinity for glutamate (Km = 72 μM) and is dependent on external Na+. The cDNA sequence predicts a protein of 543 amino acids with 6 potential transmembrane domains. Northern blot analysis indicates the mRNA encoding the glutamate transporter is expressed both in the brain and in peripheral tissues.

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