Phase-shifting effect of 8-OH-DPAT, a 5-HT1A/5-HT7 receptor agonist, on locomotor activity in golden hamster in constant darkness

doi:10.1016/0304-3940(96)12655-0

Neuroscience Letters

Volume 210, Issue 1, 24 May 1996, Pages 1-4

https://doi.org/10.1016/0304-3940(96)12655-0 Get rights and content

Abstract

The present results show that under constant darkness the endogenous circadian pacemaker located in the suprachiasmatic nuclei can be affected by administration of 8-hydroxy-2-[di-n-propylamino] tetralin (8-OH-DPAT), a well known 5-HT_1A/5-HT₇ receptor agonist. A single i.p. injection (0.1 ml) with 8-OH-DPAT (5 mg/kg) induced significant phase-advances of hamster locomotor activity at circadian time (CT) 6 and 8 and a significant phase-delay at CT11. Saline injections by themselves induced a significant phase-advance at CT10-11. The dose-response curve for 8-OH-DPAT showed a maximal phase-shifting effect for doses of at least 2.5 mg/kg at CT8. Thus, in golden hamsters, (1) 8-OH-DPAT has a chronobiological effect with sensitivity depending upon the circadian time of injection, and (2) a single saline injection is able to induce regular phase-advances at the end of the subjective day (CT10-11).

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In our hands (Experiment 4), LP-211 seems to induce a phase advancement on spontaneous circadian rhythm in mice which do not have daily temporal landmarks. In more detail, the 0.25 mg/kg dosage of LP-211 produced a phase advancement of about 6 h (that is, more than what was reported with 8–OH–DPAT in similar experimental conditions, see Cutrera et al., 1996). We propose that, compared to LP-211 which is a pure and selective agonist, 8–OH–DPAT could be characterized by a lower 5-HT(7) agonist property, thus explaining its reduced efficacy.
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^☆: This research was supported by a grant from the Commission of the European Communities, Brussels (EC contract CT-94-0036).

¹: Present address: Laboratorio de Neurociencias, Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.

²: During the present work, R.A. Cutrera was a postdoctoral fellow from EC (EC Contract C11 0636-M) and now is a permanent researcher from CONICET (National Research Council of Argentina).

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Phase-shifting effect of 8-OH-DPAT, a 5-HT1A/5-HT7 receptor agonist, on locomotor activity in golden hamster in constant darkness☆

Abstract

Brain Res.

Behav. Brain Res.

Neurosci. Lett.

Neurosci. Lett.

Brain Res.

Neuron

Brain Res.

Physiol. Behav.

Mol. Brain Res.

Brain Res. Rev.

Brain Res.

Brain Res.

Eur. J. Pharmacol.

Brain Res.

Brain Res.

Phase-shifting effect of 8-OH-DPAT, a 5-HT_1A/5-HT₇ receptor agonist, on locomotor activity in golden hamster in constant darkness☆