Elsevier

Neuroscience Letters

Volume 211, Issue 1, 14 June 1996, Pages 49-52
Neuroscience Letters

Forskolin evokes extracellular adenosine accumulation in rat cortical cultures

https://doi.org/10.1016/0304-3940(96)12718-XGet rights and content

Abstract

In this study, the effect on extracellular adenosine concentration of direct activation of adenylyl cyclase by forskolin was investigated using rat cortical cultures. Forskolin evoked intracellular and extracellular cAMP accumulation as well as extracellular adenosine accumulation. The accumulation of adenosine in response to forskolin could be blocked by the cyclic nucleotide phosphodiesterase inhibitor 4-[(3-butoxy-4-methoxyphenyl)methyl]-2-imidazolidinone (RO 20-1724; 180 μM), but not isobutylmethylxanthine (100 μM). The accumulation of adenosine in response to forskolin could be blocked by the 5′-ectonucleotidase inhibitor guanosine 5′-monophosphate. These results demonstrate that forskolin can increase extracellular adenosine levels, and that this adenosine is ultimately derived from cAMP.

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This work was supported by PHS grant NS26830 and a Mental Retardation Core Grant to Children's Hospital.

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