Elsevier

Neuroscience

Volume 63, Issue 2, November 1994, Pages 559-578
Neuroscience

Cytochrome oxidase activity in the auditory system of the mouse: A qualitative and quantitative histochemical study

https://doi.org/10.1016/0306-4522(94)90550-9Get rights and content

Abstract

Detailed qualitative and quantitative determinations of cytochrome oxidase activity in the central auditory system of BALB/cJ mice were obtained at the light microscopic level. Cytochrome oxidase activity was determined using quantitative densitometry calibrated with standards of spectrophotometrically assayed enzymatic activity. This was done together with a cobalt-intensified histochemical procedure using fresh-frozen brains without perfusion-fixation. The resulting method showed improved sensitivity and allowed quantification of histochemical labeling as actual enzyme activity units. Adjacent sections were processed for either Nissl, fiber or Golgi stains to correlate the histochemical labeling with tissue morphology. The more peripheral auditory nuclei showed primarily somatic labeling with specific cell types showing predominant reactivity. However, higher auditory structures, including the inferior colliculus, medial geniculate and auditory cortex, showed predominantly neuropil reactivity. Comparison of mean cytochrome oxidase activities for the 27 auditory regions quantified revealed a trend for decreasing activity from the brainstem to the forebrain in central lemniscal structures. The extra-lemniscal auditory regions at each level showed lower activity than the corresponding lemniscal regions. The regions with the higher activity values showed around 10 times the labeling density of the white matter, indicating the high sensitivity of the method. The darkly labeling auditory structures were clearly delineated from surrounding neural regions, supporting the concept that basal levels of oxidative metabolic capacity are larger for the auditory system.

It was concluded that the quantitative approach to cytochrome oxidase histochemistry may be applied successfully to the mouse brain. The normative data presented may be used as a starting point for other investigations of the effects of experimental manipulations on the metabolic activity of the auditory system.

References (64)

  • Gonzalez-LimaF. et al.

    Functional mapping of the rat brain during drinking behavior: a fluorodeoxyglucose study

    Physiol. Behav.

    (1993)
  • Gonzalez-LimaF. et al.

    Functional activation in the auditory system of the rat produced by arousing reticular stimulation: a 2-deoxyglucose study

    Brain Res.

    (1984)
  • Gonzalez-LimaF. et al.

    Neural substrates for tone-conditioned bradycardia demonstrated with 2-deoxyglucose. I. Activation of auditory nuclei

    Behav. Brain Res.

    (1984)
  • Gonzalez-LimaF. et al.

    Neural substrates for tone-conditioned bradycardia demonstrated with 2-deoxyglucose. II. Auditory cortex plasticity

    Behav. Brain Res.

    (1986)
  • HarrisonJ.M. et al.

    Anatomical aspects of the cochlear nucleus and superior olivary complex

  • HarveyJ.A. et al.

    Asymmetric uptake of 2-deoxy-d-[14C]glucose in the dorsal cochlear nucleus during Pavlovian conditioning in the rabbit

    Brain Res.

    (1988)
  • HessH.H. et al.

    Ultramicrospectrophotometric determination of cytochrome oxidase for quantitative histochemistry

    J. biol. Chem.

    (1953)
  • HuffmanR.F. et al.

    The descending auditory pathway and acousticomotor systems: connections with the inferior colliculus

    Brain Res. Rev.

    (1990)
  • LowryO.H. et al.

    Protein measurement with the Folin phenol reagent

    J. biol. Chem.

    (1951)
  • MastertonR.B.

    Role of the central auditory system in hearing: the new direction

    Trends Neurosci.

    (1992)
  • NobregaJ.N. et al.

    Long-term changes in regional brain cytochrome oxidase activity induced by electroconvulsive treatment in rats

    Brain Res.

    (1993)
  • SharpF.R. et al.

    Increasing intensities of wide band noise increase [14C]2-deoxyglucose uptake in gerbil central auditory structures

    Brain Res.

    (1981)
  • SilvermanM.S. et al.

    Modified technique for cytochrome oxidase histochemistry: increased staining intensity and compatability with 2-deoxyglucose autoradiography

    J. Neurosci. Meth.

    (1987)
  • Van RaamsdonkW. et al.

    Quantitative cytochemical analysis of cytochrome oxidase and succinate dehydrogenase activity in spinal neurons

    Acta histochem.

    (1987)
  • WeinbergR.J. et al.

    A cuneocochlear pathway in the rat

    Neuroscience

    (1987)
  • WillottJ.F. et al.

    Aging and presbycusis: effects on 2-deoxy-d-glucose uptake in the mouse auditory brain stem in quiet

    Expl Neurol

    (1988)
  • Wong-RileyM.

    Changes in the visual system of monocularly sutured or enucleated cats demonstrable with cytochrome oxidase histochemistry

    Brain Res.

    (1979)
  • Wong-RileyM.T.T.

    Cytochrome oxidase: an endogenous metabolic marker for neuronal activity

    Trends Neurosci.

    (1989)
  • Wong-RileyM.T.T. et al.

    Changes in endogenous enzymatic reactivity to DAB induced by neuronal inactivity

    Brain Res.

    (1978)
  • Wong-RileyM. et al.

    The effect of impulse blockage on cytochrome oxidase activity in the cat visual system

    Brain Res.

    (1983)
  • BraunK. et al.

    Distribution of parvalbumin, cytochrome oxidase activity and14C-2-deoxyglucose uptake in the brain of the zebra finch. I. Auditory and vocal motor systems

    Cell Tiss. Res.

    (1985)
  • CadaA. et al.

    Metabolic mapping of the auditory system of mice using quantitative cytochrome oxidase histochemistry

    Soc. Neurosci. Abstr.

    (1993)
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