Elsevier

Neuroscience

Volume 75, Issue 3, December 1996, Pages 815-826
Neuroscience

Metabotropic glutamate receptors mGluR2 and mGluR5 are expressed in two non-overlapping populations of Golgi cells in the rat cerebellum

https://doi.org/10.1016/0306-4522(96)00316-8Get rights and content

Abstract

The metabotropic glutamate receptor subtypes mG1uR2 and mG1uR5, which are thought to be coupled respectively to the inhibitory cyclic adenosine monophosphate (cAMP) cascade and the phosphatidylinositol hydrolysis/Ca2+ cascade, are known to be expressed on Golgi cells in the granular layer of the rat cerebellar cortex. In the present immunohistochemical study with a monoclonal antibody against mG1uR2 and a polyclonal antibody for mG1uR5, we examined whether or not mG1uR2- and mGluR5-like immunoreactivities were both present in single Golgi cells in the rat cerebellar cortex. In double immunofluorescence histochemistry, no Golgi cells showed mG1uR2- and mG1uR5-like immunoreactivities simultaneously. Of the total number of Golgi cells immunoreactive for mG1uR2 or mG1uR5, about 90% were mG1uR2-like immunoreactive, and about 10% were mG1uR5-like immunoreactive. Golgi cells with mG1uR2-like immunoreactivity were distributed evenly in the granular layer of all the cerebellar regions, while those with mG1uR5-like immunoreactivity were distributed more frequently in the 1, II, VII–X lobules of the vermis and the copula pyramidis of the hemisphere than in other cerebellar regions.

The results indicate that Golgi cells containing mG1uR2 are segregated from those possessing mG1uR5. These two populations of Golgi cells, each equipped with a different metabolic glutamate receptor coupled to a different intracellular signal transduction system, may play different roles in the glutamatergic neuronal circuits in the cerebellar cortex.

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    The expression patterns of the Group II mGluRs overlap and are both expressed in a number of brain regions and cell types, including Golgi cells of the cerebellum, dentate gyrus granule cells, the olfactory bulb, thalamus and cortex (Ohishi et al., 1993a, b; Petralia et al., 1996). mGluR2 and mGluR3 are found both in axons and dendrites of GABAergic Golgi cells in the cerebellum and in the olfactory bulb (Hayashi et al., 1993; Neki et al., 1996a, b; Ohishi et al., 1994), but in the hippocampus mGluR2 and mGluR3 predominantly labeled axons (Petralia et al., 1996; Shigemoto et al., 1997; Tamaru et al., 2001). In axons, mGluR2/3 labeling was found along the axon but largely excluded from presynaptic terminals (Shigemoto et al., 1997; Yokoi et al., 1996).

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