Preservation and visualization of actin-containing filaments in the apical zone of cochlear sensory cells
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Cited by (30)
The actin cytoskeleton in hair bundle development and hearing loss
2023, Hearing ResearchA cryo-tomography-based volumetric model of the actin core of mouse vestibular hair cell stereocilia lacking plastin 1
2020, Journal of Structural BiologyCitation Excerpt :The compression we observed of the actin core corresponds to the absence of actin-actin crosslinkers previously reported for tapers of mouse utricle stereocilia (Krey et al., 2016). In the region of the taper and rootlet, the central filaments are brought together into a tight bundle, which has been previously shown with transmission electron microscopy (Itoh, 1982; Slepecky and Chamberlain, 1982). The spacing between the filaments that we measured (~8 nm) in the rootlet region is considerably smaller than that seen through the stereocilia shaft (12–13 nm), and the filaments appear to be of a narrower diameter; these observations raise the possibility that the rootlet could be composed of a filamentous protein distinct from actin.
Actin cross-linkers and the shape of stereocilia
2010, Biophysical JournalCitation Excerpt :Finally, it yields robust structures, which is very significant because the frequency sensitivity of the ear requires a delicate regulation of the stereocilia's mechanical properties, which are in turn determined by their shape. Actin cross-linking was described early in the study of stereocilia (9) and could be responsible for the filaments being in register (10). Although cross-linkers of two types, espin and fimbrin, have been identified in stereocilia (11,12), we hereafter focus on espin, which is thought to provide sturdier cross-linking than fimbrin (13,14).
Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing
2010, CellCitation Excerpt :In contrast to actin filaments formed without GFP-TRIOBP-4, addition of GFP-TRIOBP-4 promoted organization of actin filaments into prominent bundles (Figure 3D, lower). Image analyses revealed that the spatial periodicity of actin filaments bundled with GFP-TRIOBP-4 (Figure 4A) was 8.2 ± 1.4 nm (mean ± standard deviation [SD]; n = 145), which coincides with an interfilament distance of ∼8 nm within stereocilia rootlets of guinea pig hair cells (Itoh, 1982). The distance between filaments bundled with a positive control, purified espin 3A (35 kDa) (Figure 4B), was 11.9 ± 2.1 nm (n = 148), which agrees well with 12.6 ± 0.2 nm from small-angle X-ray scattering measurements (Purdy et al., 2007).
Molecular Anatomy of Receptor Cells and Organ of Corti
2008, The Senses: A Comprehensive ReferenceAuditory hallucinations: Hypothesis in the context of spread spectrum communications
2002, Medical Hypotheses