Electrical activity, cAMP, and cytosolic calcium regulate mRNA encoding sodium channel α subunits in rat muscle cells

doi:10.1016/0896-6273(89)90190-6

Neuron

Volume 2, Issue 5, May 1989, Pages 1447-1452

https://doi.org/10.1016/0896-6273(89)90190-6 Get rights and content

Abstract

The number of sodium channels increases sharply during development of rat skeletal muscle cells in vitro. An 8.5 kb mRNA encoding sodium channel α subunit rises to a peak on day 13 in vitro and falls to a value of 50% of the peak by day 18, consistent with the conclusion that mRNA abundance is a major determinant of the rapid rise in sodium channel number. Electrical activity and increased cytosolic calcium decrease the level of α subunit mRNA, and CAMP increases its level in parallel with changes in the number of sodium channels. The similarity between the changes in mRNA levels and sodium channel density indicates that the regulation of α subunit mRNA level is an important mechanism of feedback regulation of sodium channel density by electrical activity in developing rat muscle cells.

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Neuron

ArticleElectrical activity, cAMP, and cytosolic calcium regulate mRNA encoding sodium channel α subunits in rat muscle cells

Abstract

Neuron

Biochem. Biophys. Res. Commun.

J. Biol. Chem.

J. Biol. Chem.

Neuron

J. Biol. Chem.

J. Biol. Chem.

Dev. Biol.

Neurosci. Lett.

J. Biol. Chem.

Neuron

Voltage regulated sodium channel molecules

Annu. Rev. Physiol.

Cloning and characterization of the gene for α subunit of the mammalian voltage-gated sodium channel

J. Gen. Physiol.

Probing the molecular structure of the voltage-dependent sodium channel

Annu. Rev. Neurosci.

Characteristics of saxitoxin binding to the sodium channel of sarcolemma isolated from rat skeletal muscle

J. Physiol.

The molecular basis of neuronal excitability

Science

Structure and function of voltage-sensitive ion channels

Science

Regulation of muscle acetylcholine sensitivity by muscle activity in cell culture

Science

Modulation of sodium-channel mRNA levels in rat skeletal muscle

Control of acetylcholine receptors in skeletal muscle

Physiol. Rev.

Ribonucleic acid isolated by cesium chloride centrifugation

Biochemistry

Muscle denervation increases the levels of two mRNAs coding for the acetylcholine receptor α subunit

J. Neurosci.

Voltage clamp analysis of tetrodotoxin-sensitive and -insensitive sodium channels in rat muscle cells developing in vitro

J. Neurosci.

The Conus toxin geographutoxin II distinguishes two functional sodium channel subtypes in rat muscle cells developing in vitro

J. Neurosci.

Geographutoxin-sensitive and -insensitive sodium channels in mouse skeletal muscle developing in situ

J. Physiol.

Article
Electrical activity, cAMP, and cytosolic calcium regulate mRNA encoding sodium channel α subunits in rat muscle cells