Neuron
Volume 5, Issue 2, August 1990, Pages 159-171
Journal home page for Neuron

Article
Dynamic changes in optic fiber terminal arbors lead to retinotopic map formation: An in vivo confocal microscopic study

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Abstract

Dynamic remodeling of retinal ganglion cell terminal arbors has been proposed to contribute to formation of the topographically ordered retinotectal projection. To test this directly, the growth of individual terminal arbors was observed in live X. laevis tadpoles using a confocal microscope to visualize their complex three-dimensional structure. During initial development, nasal and temporal retinal arbors covered overlapping tectal areas. Despite subsequent remodeling, the dimensions and positions of the temporal arbors remained relatively stable. In contrast, the nasal arbors grew caudally, as they extended caudal branches and retracted rostral branches. These results suggest that differences in the remodeling of the nasal and temporal arbors lead to the emergence of retino-topography along the rostrocaudal axis of the tectum. All the terminal arbors were dynamic, including those with stable dimensions, suggesting that continual remodeling of arbors may be a universal feature of neuronal projections.

References (40)

  • S.S. Easter et al.

    An evaluation of the hypothesis of shifting terminals in goldfish optic tectum

    J. Neurosci.

    (1984)
  • S.E. Fraser

    Cell interactions involved in neuronal patterning: an experimental and theoretical approach

  • S.E. Fraser et al.

    A physiological measure of shifting connections in the Rana pipiens retinotectal system

    J. Embryol. Exp. Morphol.

    (1986)
  • H. Fujisawa

    Mode of growth of retinal axons within the tectum of Xenopus tadpoles, and implications in the ordered neuronal connection between the retina and the tectum

    J. Comp. Neurol.

    (1987)
  • R.M. Gaze et al.

    The evolution of the retinotectal map during development in Xenopus

  • R.M. Gaze et al.

    The relationship between retinal and tectal growth in larval Xenopus: implications for the development of the retinotectal projection

    J. Embryol. Exp. Morphol.

    (1979)
  • W.A. Harris et al.

    Retinal axons with and without their somata, growing to and arborizing in the tectum of Xenopus embryos: a time lapse video study of single fibres in vivo

    Development

    (1987)
  • C.D. Heffner et al.

    Target control of collateral initiation and directional axon growth in the mammalian brain

    Science

    (1990)
  • C.E. Holt

    A single-cell analysis of early retinal ganglion cell differentiation in Xenopus: from soma to axon tip

    J. Neurosci.

    (1989)
  • C.E. Holt et al.

    Order in the initial retinotectal map in Xenopus: a new technique for labeling growing nerve fibres

    Nature

    (1983)
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    Present address: Department of Biological Sciences, Stanford University, Stanford, California 94305-5020.

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