Elsevier

Brain Research

Volume 901, Issues 1–2, 18 May 2001, Pages 277-280
Brain Research

Short communication
CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge

https://doi.org/10.1016/S0006-8993(01)02365-4Get rights and content

Abstract

The expression of cholecystokinin (CCK) mRNA in neuroendocrine corticotropin-releasing hormone (CRH) neurons of the hypothalamic paraventricular nucleus (PVN) of male rats was examined 8 h following an acute immune challenge by intraperitoneal lipopolysaccharide (LPS, 250 μg/kg). Both quantitative, macroautoradiographic, single-label radioactive in situ hybridization histochemistry (ISHH) and qualitative dual-label ISHH were performed. Compared to controls, LPS-injected rats displayed increased (185%) parvicellular CCK mRNA expression levels, occurring in a majority (70%) of CRH neurons as revealed by dual-label ISHH.

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Acknowledgements

We thank M.C. Fournier in Professor B. Bloch’s laboratory (University of Bordeaux II), for linearization of the plasmids, M. Chaigniau for illustrations and C. Fargues for secretarial skills. The CRH and CCK cDNAs were kindly provided by Dr P.E. Sawchenko (Salk Institute, La Jolla), and Dr M. Pohl (INSERM, Paris), respectively.

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