Sleep Deprivation by the “Flower Pot” Technique and Spatial Reference Memory
Section snippets
Methods
In a pilot study (data not reported here), 400-g rats were housed in cages with platforms of surface area 33 cm2 for SD and 154 cm2 for TC rats. Both TC and SD rats lost significant amounts of weight during the 5 days of the experiment, and 1 SD rat died on Day 4. In addition, TC rats exhibited increased locomotor activity by swimming faster than either CC or SD rats, and also showed partial impairment of reference memory, compared with CC rats, suggesting that the ratio of rat size to platform
Results
Initial body weights, weight change, and adrenal weights of the rats are given in Table 1. SD rats lost weight, TC rats maintained weight, and CC rats gained weight during the 4-day experiment. The weight change of SD rats was significantly different from that of CC or TC rats [F(2,20) = 56, p < 0.0001]. Treatment had no significant effect on adrenal gland weight and none of the rats had visually obvious gastric ulcers. Serum corticosterone was significantly increased in SD rats compared with
Discussion
This study demonstrates that 4 days of SD stress causes a substantial impairment of reference memory, but has no effect on working memory in rats. These results agree with those of Stern [28], who demonstrated that SD rats were able to retain a passive-avoidance behavior when tested 5 s following a single training session, but showed a deterioration of retention when tested 5 min after training. The time intervals used in this study were 30 s between each trial and approximately 25 min between
Acknowledgements
This work was supported by the U.S. Army Medical Research and Development Commmand grant number DAMD 17-92-V-2009.
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