[22] Use of drugs to study role of microtubule assembly dynamics in living cells
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2017, Seminars in Cell and Developmental BiologyCitation Excerpt :A comparable mechanism has been described for mammalian MACF1 [68,106]. We found that shot mutant neurons, treated with the MT-stabilising drug taxol (promoting MT polymerisation, stabilisation and bundling) [107], display a shift of MTs to the distal end of axons (N.S.S., unpublished data; Fig. 4G, H). This phenotype might be caused by lack of minus end anchorage of MTs, in combination with axonal MT sliding performed by kinesins [108,109].
The role of microtubules and their dynamics in cell migration
2012, Journal of Biological ChemistryEndocytic pathway rapidly delivers internalized molecules to lysosomes: An analysis of vesicle trafficking, clustering and mass transfer
2012, Journal of Controlled ReleaseCitation Excerpt :Surprisingly, however, overexpression of constitutive active Rab-7 (Q67L) [14] also led to reduced mass transfer. Other cases, including direct inhibition of Rab-7 using siRNA, use of lower pH to promote vesicle clustering [30], addition of BAPTA to inhibit clustering [31] and incubation of cells with nocodazole to inhibit microtubular transport [32], all led to reduced mass transfer rate. The different conditions used in this study altered the total uptake widely from about 300–400 vesicles per cell in case of control to about 50 in the presence of BAPTA.
New insights into mechanisms of resistance to microtubule inhibitors
2011, Biochimica et Biophysica Acta - Reviews on CancerMutant huntingtin alters cell fate in response to microtubule depolymerization via the GEF-H1-RhoA-ERK pathway
2010, Journal of Biological ChemistryCitation Excerpt :Pdx depolymerized MT between 0.5 and 1 h (supplemental Fig. S2 and Fig. 1D) at concentrations that rescued cell death (Fig. 1E). The EC50 for rescue was comparable with the reported EC50 for MT depolymerization by these compounds (∼25 nm for Pdx) (24). We confirmed rescue using three independent cell viability assays (Fig. 1, C, E, and F).